Effect of the 3'APOB-VNTR polymorphism on the lipid profiles in the Guangxi Hei Yi Zhuang and Han populations.
Effect of the 3'APOB-VNTR polymorphism on the lipid profiles in the Guangxi Hei Yi Zhuang and Han populations.
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DOI：
10.1186/1471-2350-8-45
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发表时间：
2007-07-17
影响因子：
--
通讯作者：
Shangling P
中科院分区：
文献类型：
--
作者：
Ruixing Y;Guangqin C;Yong W;Weixiong L;Dezhai Y;Shangling P
载脂蛋白（Apo）B是低密度脂蛋白（LDL）、极低密度脂蛋白（VLDL）和乳糜微粒的主要成分。载脂蛋白B的许多遗传多态性已被描述，与脂质水平的变化。然而，在中国少数民族人群中，关于载脂蛋白B基因3'端可变数目串联重复序列区（3' APOB-VNTR）多态性对血脂谱的影响的研究很少。因此，本研究旨在探讨广西黑衣壮、汉族人群APOB-VNTR基因3 ′端多态性与血脂水平的关系。采用分层随机整群抽样方法，对黑衣壮548人进行调查。流行病学调查采用国际标准化方法进行。测定血脂和载脂蛋白水平。采用聚合酶链反应（PCR）和聚丙烯酰胺凝胶电泳技术检测3 'APOB-VNTR等位基因，并根据15-bp高变元件（HVE）的重复数进行分类。最常见的等位基因的序列使用PCR和直接测序确定。检测了3 'APOB-VNTR等位基因与血脂变量之间的可能关联。并与496名汉族人的结果进行了比较。在黑衣壮、汉族人群中共检测到19个等位基因，重复数在24 ~ 64之间。HVE 56和HVE 58在黑衣壮人群中未检测到，HVE 48和HVE 62在汉族人群中完全缺失。HVE 26、HVE 30、HVE 46、杂合子和短等位基因（< 38个重复）的频率在黑衣壮人群中高于汉族人群。而HVE 34、HVE 38、HVE 40、纯合子和长等位基因（≥ 38个重复）频率在黑衣壮组低于汉族组（P < 0.05-0.01）。黑衣壮人群中VNTR-LS（携带一长一短等位基因）的总胆固醇（TC）、高密度脂蛋白胆固醇（HDL-C）和载脂蛋白B水平高于VNTR-LL（携带两长等位基因）。HVE 34和HVE 36等位基因的黑依壮人TC、TG、LDL胆固醇和Apo B水平均高于HVE 32等位基因。黑衣壮纯合子的TC、TG、HDL-C和Apo B水平也高于杂合子。在两个民族中，VNTR-SS（携带两个短等位基因）与VNTR-LS或VNTR-LL基因型之间的血脂检测指标无显著差异。黑衣壮、汉族人群3 'APOB-VNTR多态性存在显著差异。在黑衣壮人群中，APOB-VNTR基因多态性与血脂水平相关，而在汉族人群中与血脂水平无关。
Apolipoprotein (Apo) B is the major component of low-density lipoprotein (LDL), very low-density lipoprotein (VLDL) and chylomicrons. Many genetic polymorphisms of the Apo B have been described, associated with variation of lipid levels. However, very few studies have evaluated the effect of the variable number of tandem repeats region 3' of the Apo B gene (3'APOB-VNTR) polymorphism on the lipid profiles in the special minority subgroups in China. Thus, the present study was undertaken to study the effect of the 3'APOB-VNTR polymorphism on the serum lipid levels in the Guangxi Hei Yi Zhuang and Han populations. A total of 548 people of Hei Yi Zhuang were surveyed by a stratified randomized cluster sampling. The epidemiological survey was performed using internationally standardized methods. Serum lipid and apolipoprotein levels were measured. The 3'APOB-VNTR alleles were determined by polymerase chain reaction (PCR) followed by electrophoresis in polyacrylamide gels, and classified according to the number of repeats of a 15-bp hypervariable elements (HVE). The sequence of the most common allele was determined using the PCR and direct sequencing. The possible association between alleles of the 3'APOB-VNTR and lipid variables was examined. The results were compared with those in 496 people of Han who also live in that district. Nineteen alleles ranging from 24 to 64 repeats were detected in both Hei Yi Zhuang and Han. HVE56 and HVE58 were not be detected in Hei Yi Zhuang whereas HVE48 and HVE62 were totally absent in Han. The frequencies of HVE26, HVE30, HVE46, heterozygote, and short alleles (< 38 repeats) were higher in Hei Yi Zhuang than in Han. But the frequencies of HVE34, HVE38, HVE40, homozygote, and long alleles (≥ 38 repeats) were lower in Hei Yi Zhuang than in Han (P < 0.05–0.01). The levels of total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C) and Apo B in Hei Yi Zhuang but not in Han were higher in VNTR-LS (carrier of one long and one short alleles) than in VNTR-LL (the individual carrying two long alleles) genotypes. The levels of TC, triglycerides (TG), LDL cholesterol, and Apo B in Hei Yi Zhuang were higher in both HVE34 and HVE36 alleles than in HVE32 allele. The levels of TC, TG, HDL-C and Apo B in Hei Yi Zhuang were also higher in homozygotes than in heterozygotes. There were no significant differences in the detected lipid parameters between the VNTR-SS (carrier of two short alleles) and VNTR-LS or VNTR-LL genotypes in both ethnic groups. There were significant differences of the 3'APOB-VNTR polymorphism between the Hei Yi Zhuang and Han populations. An association between the 3'APOB-VNTR polymorphism and serum lipid levels was observed in the Hei Yi Zhuang but not in the Han populations.