Immediate-early transcriptional regulation and rapid mRNA turnover of a putative serine/threonine protein kinase.
Immediate-early transcriptional regulation and rapid mRNA turnover of a putative serine/threonine protein kinase.
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DOI：
10.1016/s0021-9258(19)50221-6
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发表时间：
1993-06
期刊：
影响因子：
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通讯作者：
Melanie K. Webster;Luis Goya;G. Firestone
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文献类型：
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作者：
Melanie K. Webster;Luis Goya;G. Firestone
丝氨酸/苏氨酸蛋白激酶是包括代谢、增殖和分化在内的多种细胞过程的重要调节剂。我们以前已经确定了一个49 kDa的丝氨酸/苏氨酸激酶，指定的sgk，这是转录响应糖皮质激素激素和血清在上皮细胞的cDNA。我们在这里报告说，sgk表达也迅速诱导地塞米松或血清在大鼠2成纤维细胞。核Run-on和北方印迹分析显示，sgk mRNA的诱导是静止成纤维细胞对血清刺激的立即早期转录反应，其发生在c-jun转录的峰值之后。与糖皮质激素刺激的大鼠2成纤维细胞中的sgk表达相反，血清的sgk的转录诱导是短暂的，sgk的转录本衰变，特别是快速的半衰期为20分钟。sgk的快速营业额，结合其即时早期的转录响应血清，提示了一种新的机制，在G 0到S的过渡和进入细胞周期的促有丝分裂信号。
Serine/threonine protein kinases are important regulators of diverse cellular processes including metabolism, proliferation, and differentiation. We have previously identified the cDNA for a 49-kDa serine/threonine kinase, designated sgk, which is transcriptionally responsive to glucocorticoid hormones and serum in epithelial cells. We report here that sgk expression is also rapidly induced by dexamethasone or serum in Rat2 fibroblasts. Nuclear run-on and Northern blot analysis revealed that the induction of sgk mRNA is an immediate-early transcriptional response to serum stimulation of quiescent fibroblasts, which occurs just after the peak in c-jun transcription. In contrast to the glucocorticoid-stimulated sgk expression in Rat2 fibroblasts, the transcriptional induction of sgk by serum was transient and sgk transcripts decayed with a particularly rapid half-life of 20 min. The rapid turnover of sgk, in combination with its immediate-early transcriptional response to serum, suggests a novel mechanism for responding to mitogenic signals during G0 to S transition and entry into the cell cycle.