Longitudinal characterization of the IgM and IgG humoral response in symptomatic COVID-19 patients using the Abbott Architect.
Longitudinal characterization of the IgM and IgG humoral response in symptomatic COVID-19 patients using the Abbott Architect.
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使用Abbott Architect的有症状的Covid-19患者中IgM和IgG体液反应的纵向表征。
DOI：
10.1016/j.jcv.2020.104663
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发表时间：
2020-12
期刊：
影响因子：
--
通讯作者：
Sun Q
中科院分区：
文献类型：
--
作者：
Maine GN;Lao KM;Krishnan SM;Afolayan-Oloye O;Fatemi S;Kumar S;VanHorn L;Hurand A;Sykes E;Sun Q
与IgG相比，针对SARS-CoV-2的Abbott IgM检测检测时间稍早。雅培IgM和IgG检测均表现出良好的敏感性和特异性。症状出现3个月后IgM阳性率降至30.8%，而症状出现3 - 6个月后IgG阳性率为92.3%。在PCR检测SARS-CoV-2感染呈阴性的有症状患者中，评估IgM和IgG抗体有助于支持COVID-19的诊断。抗体检测最近成为协助确定COVID-19病原体SARS-CoV-2暴露的一种选择。阐明体液反应的动力学和持续时间对临床管理和解释血清学调查结果很重要。在此，我们评估了雅培SARS-CoV-2 IgM和IgG检测的临床表现，以及有症状的COVID-19患者抗体反应的纵向动态。使用300份covid -19前血清标本，IgM的诊断特异性为100%，IgG的诊断特异性为99.67%。使用从427例pcr确诊个体中收集的1349份连续血清样本，在症状出现后168天内，SARS-CoV-2 IgM检测的临床试验灵敏度在≤7天为24.6%，在8 ~ 14天为75.3%，在15 ~ 21天为95.0%，在4 ~ 5周为96.0%（试验灵敏度峰值）。IgM血清转化的中位持续时间为10天。症状出现后4 ~ 5周IgM水平稳步下降，3个月时阳性率降至30.8%。SARS-CoV-2 IgG检测在症状出现后≤7天的诊断敏感性为23.2%，8 ~ 14天为69.5%，15 ~ 21天为93.6%，4 ~ 5周为99.6%（检测敏感性峰值）。IgG血清转化的中位持续时间为11.5天。在感染恢复期，观察患者IgG水平下降，随访100天。尽管有所下降，但92.3%的患者在症状出现后3-6个月仍保持IgG阳性。本研究表明，与IgG相比，针对SARS-CoV-2的Abbott IgM检测方法检测时间稍早，两种检测方法均具有出色的总体敏感性和特异性。在PCR检测SARS-CoV-2感染呈阴性的有症状患者中，评估IgM和IgG抗体有助于支持COVID-19的诊断。
The Abbott IgM assay against SARS-CoV-2 is detected slightly earlier compared to IgG. Both Abbott IgM and IgG assays exhibit excellent sensitivity and specificity. Positive rate of IgM drops to 30.8 % after 3 months of symptoms, in contrast to IgG which is sustained in 92.3 % patients 3–6 months post symptom onset. In symptomatic patients who test negative by PCR for a SARS-CoV-2 infection, assessing IgM and IgG antibodies can aid in supporting a diagnosis of COVID-19. Antibody testing has recently emerged as an option to assist with determining exposure to SARS-CoV-2, the causative agent of COVID-19. Elucidation of the kinetics and duration of the humoral response is important for clinical management and interpreting results from serological surveys. Here we evaluated the clinical performance of Abbott SARS-CoV-2 IgM and IgG assays, as well as the longitudinal dynamics of the antibody response in symptomatic COVID-19 patients. The diagnostic specificity was 100 % for IgM and 99.67 % for IgG using 300 pre-COVID-19 serum specimens. Using 1349 sequential serum samples collected up to 168 days post symptom onset from 427 PCR-confirmed individuals, clinical test sensitivity of the SARS-CoV-2 IgM assay was 24.6 % at ≤7 days, 75.3 % at 8−14 days, 95.0 % at 15−21 days, and 96.0 % at 4−5 weeks (peak test sensitivity). The median duration of time for IgM seroconversion was 10 days. IgM levels declined steadily 4−5 weeks after symptom onset, and the positive rate dropped to 30.8 % at >3 months. The diagnostic sensitivity for the SARS-CoV-2 IgG assay post symptom onset was 23.2 % at ≤7 days, 69.5 % at 8−14 days, 93.6 % at 15−21 days, and 99.6 % at 4−5 weeks (peak test sensitivity). The median duration of time for IgG seroconversion was 11.5 days. During the convalescent phase of the infection, a decline in the IgG level was observed in patients who were followed for >100 days. Despite that decline, 92.3 % of the patient cohort remained IgG positive 3–6 months following symptom onset. This study demonstrates the Abbott IgM assay against SARS-CoV-2 is detected slightly earlier compared to IgG, with both tests exhibiting excellent overall sensitivity and specificity. In symptomatic patients who test negative by PCR for a SARS-CoV-2 infection, assessing IgM and IgG antibodies can aid in supporting a diagnosis of COVID-19.
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影响因子：
2
作者：
Zhao, Jijun;Wang, Qin;Zhang, Jianhua
通讯作者：
Zhang, Jianhua
影响因子：
6.9
作者：
Mo, HY;Zeng, GQ;Zhong, NS
通讯作者：
Zhong, NS
DOI：
10.1128/cdli.11.4.665-668.2004
发表时间：
2004-07-01
期刊：
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
影响因子：
--
作者：
Woo, PCY;Lau, SKP;Yuen, KY
通讯作者：
Yuen, KY
影响因子：
24.8
作者：
Premkumar, Lakshmanane;Segovia-Chumbez, Bruno;de Silva, Aravinda M.
通讯作者：
de Silva, Aravinda M.
影响因子：
11.8
作者：
Hanson, Kimberly E.;Altayar, Osama;Mustafa, Reem A.
通讯作者：
Mustafa, Reem A.