Glycine receptor α4 subunit facilitates the early embryonic development in mice
Glycine receptor α4 subunit facilitates the early embryonic development in mice
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DOI：
10.1530/rep-19-0312
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发表时间：
2020-01-01
期刊：
影响因子：
3.8
通讯作者：
Yasuda, Ryohei
中科院分区：
文献类型：
--
作者：
Nishizono, Hirofumi;Darwish, Mohamed;Yasuda, Ryohei
输卵管积液对于受精和随后的植入前发育是必不可少的。甘氨酸在输卵管液中含量丰富，据报道对哺乳动物受精卵的植入前发育至关重要。然而，甘氨酸对受精卵发挥作用的机制尚不清楚。在这里，我们发现甘氨酸通过甘氨酸受体调节小鼠受精卵的着床前发育。其中，α-4亚基(Glra4)和β亚基在小鼠受精卵中表达，缺乏Glra4会抑制胚胎发育到囊胚期，减少囊胚中的细胞数和产仔数。因此，我们发现了甘氨酸受体的一个新功能，它被认为主要作为神经递质受体调节胚胎发育，我们的数据为输卵管环境与哺乳动物受精卵之间的相互作用提供了新的见解。
Oviduct fluid is essential for the fertilization and subsequent preimplantation development. Glycine is abundant in oviduct fluid and is reported to be critical for preimplantation development of fertilized eggs in mammals. However, the mechanism by which glycine exerts its action on fertilized eggs is yet to be understood. Here we show that glycine regulates the preimplantation development of mouse fertilized eggs via glycine receptors. Among them, the alpha-4 subunit (Glra4) and the beta subunit are expressed in mouse fertilized eggs, and lacking Glra4 inhibits embryonic development to the blastocyst stage, decreases the number of cells in the blastocysts and the litter size. Thus, we identify a novel function of the glycine receptor, which is considered to act mainly as a neurotransmitter receptor, as a regulator of embryonic development and our data provide new insights into the interactions between oviduct milieu and mammalian fertilized egg.