Near-Full-Length Genome Sequences of a Novel HIV-1 Circulating Recombinant Form, CRF01_AE/B'/C (CRF78_cpx), in Yunnan, China.
Near-Full-Length Genome Sequences of a Novel HIV-1 Circulating Recombinant Form, CRF01_AE/B'/C (CRF78_cpx), in Yunnan, China.
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DOI：
10.1089/aid.2015.0351
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发表时间：
2016-05
影响因子：
1.5
通讯作者：
Yindi Song;Yue Feng;Zhijiang Miao;Binghui Wang;Ming Yang;A. Zhang;Li Liu;X. Xia
中科院分区：
文献类型：
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作者：
Yindi Song;Yue Feng;Zhijiang Miao;Binghui Wang;Ming Yang;A. Zhang;Li Liu;X. Xia
我们报告了一种新的HIV循环重组体（CRF 78_cpx），主要由CRF 01_AE和来自中国云南省3个流行病学上无关联的个体的B和C亚型插入片段组成。其中两名受试者是异性恋男性，一名是男性静脉注射吸毒者。对这三个分离株（YNTC 88、YNTC 19和YNTC 35）的近全长基因组进行测序和分析，发现所有三种病毒的重组断点相同，但它们之间在基因组上存在相当大的遗传多样性，这表明这不是新产生的CRF，只是新检测到的。CRF78_cpx在其独特的骨架、插入片段大小和断点方面不同于先前记录的CRF 01-AE/B '/C形式，并且与区域中的其他描述的重组体如CRF07_BC或CRF65_cpx（也由CRF01_AE、B'和C组成）无关。我们目前的研究结果进一步丰富了云南流行的HIV-1 CRF的多样性，云南被认为是中国HIV-1感染的中心。
We report a novel HIV circulating recombinant form (CRF78_cpx) composed mainly of CRF01_AE with inserts from subtypes B and C identified from three epidemiologically unlinked individuals in Yunnan province, China. Two of the subjects are heterosexual men and one is a male intravenous drug user. Sequencing and analyzing the near-full-length genome of these three isolates (YNTC88, YNTC19, and YNTC35) revealed identical recombination breakpoints in all three viruses, but considerable genetic diversity between them, across the genomes, indicating that this is not a newly created CRF, only newly detected. CRF78_cpx differs from previously documented CRF01-AE/B'/C forms in its distinct backbone, inserted fragment size, and breakpoints, and is not related to other described recombinants in the region such as CRF07_BC or CRF65_cpx (also composed of CRF01_AE, B', and C). Our present findings further enrich the diversity of the prevalent HIV-1 CRFs in Yunnan, which is considered as an epicenter of HIV-1 infections in China.