The Relationship between Cutaneous Wounds Made on Obese Mice or Those with Decreased Body Weight and Serum Leptin Level
The Relationship between Cutaneous Wounds Made on Obese Mice or Those with Decreased Body Weight and Serum Leptin Level
复制标题
DOI：
10.4236/health.2016.811105
复制
发表时间：
2016-08
期刊：
影响因子：
2.1
通讯作者：
T. Urai;Haryanto;Kanae Mukai;T. Matsushita;Kimi Asano;Yukari Nakajima;M. Okuwa;J. Sugama;T. Nakatani
中科院分区：
文献类型：
--
作者：
T. Urai;Haryanto;Kanae Mukai;T. Matsushita;Kimi Asano;Yukari Nakajima;M. Okuwa;J. Sugama;T. Nakatani
目的：并非所有肥胖者都有高血糖症。我们想知道没有高血糖的肥胖者的康复进展。本研究的目的是观察皮肤创面的愈合过程。方法：3周龄雄性小鼠随意饲喂高脂饲料（脂肪含量60%），对照组饲喂商品饲料15周。在小鼠背部皮肤上制作圆形全层皮肤创面。从伤口愈合后第0天到第15天，我们分析伤口愈合过程。我们测量了血中瘦素的浓度，并观察了瘦素阳性细胞在每个创面的分布。结果：饲喂15周时，饲粮组的平均体重、皮下脂肪面积、内脏脂肪面积、附睾脂肪重量均显著大于对照组。饮食组伤后不进食；他们的体重明显下降到对照组的水平。每天饮食组和对照组的伤口面积、再上皮化和胶原纤维的比例没有差异。饮食组伤前及伤后第6 d血清瘦素浓度均显著高于对照组（伤后第0、10 h和第1 d: p < 0.01，第6 d: p < 0.05）。结论：肥胖小鼠与非肥胖小鼠的伤口愈合过程相似，肥胖小鼠体内瘦素水平较非肥胖小鼠的下降可能与肥胖小鼠体重的减轻有关。
Purpose: Not all obese people have hyperglycemia. We wondered about the healing progress in obese people without hyperglycemia. The purpose of this study is to observe the cutaneous wound healing process. Methods: Three-week-old male mice were fed high-fat diets (containing 60% fat) in the diet group, and commercial diets in the control group, ad libitum for 15 weeks. Circle-full-thickness cutaneous wounds were made on the dorsal skin of mice. From day 0 to day 15 after wounding, we analyzed wound healing process. We measured the blood concentration of leptin, and observed the distribution of leptin-positive cells in each wound. Results: Mean body weight, the areas of subcutaneous fat and visceral fat, and the weight of epididymal fat in the diet group were significantly greater than those in the control group at 15 weeks after feeding. The diet group did not feed on the diet after wounding; their body weight decreased remarkably to the level of the control group. The ratio of wound area, re-epithelialization, and collagen fibers did not differ between the diet and control groups on each day. The blood concentration of leptin in the diet group was significantly greater than that in the control group before wounding and until day 6 after wounding (day 0, 10 hour and day 1: p < 0.01, day 6: p < 0.05). Conclusion: The results show that the wound healing process is similar between obese and non-obese mice, and that the decrease in the leptin level in the obese mouse to that in the non-obese mouse may depend on the decrease of body weight of obese mouse.