Detection of "Rickettsia sp strain Uilenbergi" and "Rickettsia sp strain Davousti" in Amblyomma tholloni ticks from elephants in Africa
Detection of "Rickettsia sp strain Uilenbergi" and "Rickettsia sp strain Davousti" in Amblyomma tholloni ticks from elephants in Africa
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DOI：
10.1186/1471-2180-7-74
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发表时间：
2007-08-07
期刊：
影响因子：
4.2
通讯作者：
Raoult, Didier
中科院分区：
文献类型：
--
作者：
Matsumoto, Kotaro;Parola, Philippe;Raoult, Didier
背景：到目前为止，已知有6种硬质立克次体在非洲发生，其中4种在被确认为人类病原体之前首次在硬壳中被鉴定。结果：通过对立克次体属部分GLTA和OmpA基因的部分PCR扩增和测序，我们分别检测了中非共和国和加蓬的33只和5只索洛隆Amblyomma tholLoni硬蜱。从加蓬硬蜱中检测到的GLTA和OmpA基因部分序列与黑龙江立克次体的同源性为99.1%，与立克次体的同源性为97.1%。HL-93菌株。在中非共和国发现的部分GLTA和OmpA基因序列与立克次体的同源性分别为98.9%和95.1%。DnS14菌株和马氏菌。系统发育分析表明，立克次体为Rickettsia sp.在基于GLTA和OmpA部分基因的系统发育树中，在加蓬发现了与日本大黄和黑龙江大黄的聚集性。立克次体的基因分型。在中非共和国发现的立克次体与马氏立克次体在基于GLTA基因部分序列的系统发育树上的相似性较近，而在基于OmpA部分基因的系统发育树上与其他立克次体的亲缘关系较远。结论：部分GLTA和OmpA基因与已知物种的相似度表明，本研究检测到的立克次体可能是新种，尽管我们只能从DNA含量上研究2个基因的部分序列。我们提出了立克次体。在加蓬发现的立克次体暂定名为“Rickettsia sp.straint Davousti”和Rickettsia sp.将在中非共和国发现的病毒命名为“立克次体菌株Uilenbergi”。
Background: To date, 6 tick-borne rickettsiae pathogenic for humans are known to occur in Africa and 4 of them were first identified in ticks before being recognized as human pathogens.Results: We examined 33 and 5 Amblyomma tholloni ticks from African elephants in the Central African Republic and Gabon, respectively, by PCR amplification and sequencing of a part of gltA and ompA genes of the genus Rickettsia. The partial sequences of gltA and ompA genes detected in tick in Gabon had 99.1% similarity with those of R. heilongjiangensis and 97.1% with those of Rickettsia sp. HL-93 strain, respectively. The partial gltA and ompA gene sequences detected in tick in the Central African Republic were 98.9% and 95.1% similar to those of Rickettsia sp. DnS14 strain and R. massiliae, respectively. Phylogenetic analysis showed Rickettsia sp. detected in Gabon clusters with R. japonica and R. heilongjiangensis in a phylogenetic tree based on the partial gltA and ompA genes. The genotype of the Rickettsia sp. detected in the Central African Republic is close to those of R. massiliae group in the phylogenetic tree based on partial gltA gene sequences, and distantly related to other rickettsiae in the tree based on partial ompA gene.Conclusion: The degrees of similarity of partial gltA and ompA genes with recognized species indicate the rickettsiae detected in this study may be new species although we could only study the partial sequences of 2 genes regarding the amount of DNA that was available. We propose the Rickettsia sp. detected in Gabon be provisionally named "Rickettsia sp. stain Davousti" and Rickettsia sp. detected in the Central African Republic be named "Rickettsia sp. strain Uilenbergi".