Identification of urinary Gc-globulin as a novel biomarker for bladder cancer by two-dimensional fluorescent differential gel electrophoresis (2D-DIGE)
Identification of urinary Gc-globulin as a novel biomarker for bladder cancer by two-dimensional fluorescent differential gel electrophoresis (2D-DIGE)
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通过二维荧光微分凝胶电泳 (2D-DIGE) 鉴定尿 Gc 球蛋白作为膀胱癌的新型生物标志物
DOI：
10.1016/j.jprot.2012.09.002
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发表时间：
2012-12-21
影响因子：
3.3
通讯作者：
Tan, Wan-long
中科院分区：
文献类型：
--
作者：
Li, Fei;Chen, Ding-nan;Tan, Wan-long
提高膀胱癌的早期发现率和监测水平仍然是医学界面临的一大挑战。在这里，我们用二维荧光差示凝胶电泳法(2D-DGE)和基质辅助激光解吸飞行时间质谱仪(MALDI-TOF/TOF MS)鉴定了膀胱癌患者和正常对照组尿中包括GC-球蛋白(GC)在内的16种蛋白质。生物信息学分析表明，GC在生长、凋亡、死亡和表皮生长因子受体活性的调节中起着重要作用。用Western blotting、免疫组织化学染色和酶联免疫吸附试验(EL ISA)进一步定量检测病例和对照组尿液或组织中GC的表达模式。经肌酐校正后的EL ISA定量结果显示，膀胱癌患者的GC-Cr水平显著高于良性膀胱病变患者和正常对照组(分别为1013.70±851.25和105.32±47.81 ng/mg)。受试者工作特性分析显示，以161.086 ng/mg尿液GC检测膀胱癌的敏感度为92.31%，特异度为83.02%；1407.481 ng/mg尿液GC诊断浸润性尿路上皮癌的敏感度为82.61%，特异度为88.24%。综上所述，我们确定GC是一种潜在的新的尿液生物标志物，用于膀胱癌的早期发现和监测。(C)2012爱思唯尔B.V.保留所有权利。
Improving the early detection rate and surveillance of bladder cancer remains a great challenge in medicine. Here, we identified sixteen proteins including Gc-globulin (GC) in urine from bladder cancer patients and normal controls by two-dimensional fluorescent differential gel electrophoresis (2D-DIGE) and matrix-assisted laser desorption time-of-flight mass spectrometry (MALDI-TOF/TOF MS). Bioinformatics analyses indicated GC played important roles in the regulation of growth, apoptosis, death and epidermal growth factor receptor activity. The GC expression patterns in urine or tissue from cases and controls were further quantified by western blotting, immunohistochemical staining and enzyme-linked immunosorbent assay (ELISA). ELISA quantification by correcting for creatinine expression showed GC-Cr was significantly increased in bladder cancer patients than in benign bladder damages cases and normal controls (1013.70 +/- 851.25 versus 99.34 +/- 55.87, 105.32 +/- 47.81 ng/mg, respectively). Receiver operating characteristic (ROC) analysis suggested that at 161.086 ng/mg urinary GC, bladder cancer could be detected with 92.31% sensitivity and 83.02% specificity, and 1407.481 ng/mg with 82.61% sensitivity and 88.24% specificity could be used for the detection of infiltrating urothelial carcinoma of bladder cancer. Taken together, we identified GC as a potential novel urinary biomarker for the early detection and surveillance of bladder cancer. (C) 2012 Elsevier B.V. All rights reserved.