Antiviral effect of prostaglandins of the A series: inhibition of vaccinia virus replication in cultured cells.
Antiviral effect of prostaglandins of the A series: inhibition of vaccinia virus replication in cultured cells.
复制标题
A系列前列腺素的抗病毒作用：抑制培养细胞中痘苗病毒的复制。
DOI：
10.1099/0022-1317-63-2-435
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Esteban,M
中科院分区：
文献类型：
--
作者：
Santoro,MG;Jaffe,BM;Garaci,E;Esteban,M
A系列前列腺素有效地抑制小鼠L成纤维细胞中牛痘病毒的产生。在最高无毒剂量的PGA 1（4 µg/ml）下，病毒复制抑制率为95.3%。抗病毒活性是剂量依赖性的，并且对A系列具有特异性。在使用的剂量下，PGA 1对未感染的细胞没有毒性，并且通过DNA、RNA和蛋白质合成测量，没有改变细胞代谢。PGA 1不影响宿主细胞对病毒的吸附，在感染的早期阶段，抗病毒活性不依赖于PGA 1的存在。PGA治疗延迟和部分抑制病毒DNA的合成，而它没有产生任何变化的蛋白质合成模式在未感染的细胞，它改变了病毒蛋白质合成的速率和模式。我们的结论是，PGA 1选择性地抑制一个或多个步骤参与牛痘病毒在小鼠L成纤维细胞的复制。
Prostaglandins of the A series potently inhibited the production of vaccinia virus in mouse L fibroblasts. With the highest non-toxic dose of PGA1, 4 µg/ml, the replication of the virus was inhibited by 95.3%. The antiviral activity was dose-dependent and specific for the A series. At the dose used, PGA1was not toxic to uninfected cells and did not alter cell metabolism as measured by DNA, RNA and protein synthesis. PGA1did not influence the adsorption of the virus by the host cells and the antiviral activity was not dependent on the presence of PGA1during the early stages of infection. PGA treatment delayed and partially inhibited virus DNA synthesis and, while it did not produce any change in the pattern of protein synthesis in uninfected cells, it altered both the rate and the pattern of virus protein synthesis. We conclude that PGA1selectively inhibits one or more steps involved in the replication of vaccinia virus in mouse L fibroblasts.