Establishment of an Experimental Mouse Model of Trauma-Hemorrhagic Shock
Establishment of an Experimental Mouse Model of Trauma-Hemorrhagic Shock
复制标题
小鼠创伤失血性休克实验模型的建立
DOI：
10.1538/expanim.61.417
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发表时间：
2012-07-01
影响因子：
2.4
通讯作者：
Liang, Ting-Bo
中科院分区：
文献类型：
--
作者：
Tang, Yin;Xia, Xue-Feng;Liang, Ting-Bo
本研究建立了创伤失血性休克（THS）小鼠模型。THS诱导小鼠（C57 BL/6 J，n=33）股骨骨折，缺血90 min，复苏15 min。假手术小鼠（C57 BL/6 J，n=33）进行相同的麻醉和手术程序，但不进行创伤出血和液体复苏。在所有小鼠中纵向测量小肠、肝脏和左肾的平均动脉压（MAP）和微血管组织灌注。在基线和复苏后3、6、12和24小时收集血液用于分析，并在复苏后24小时切除小肠、肝脏和左肾用于苏木精和伊红染色。与假手术组相比，THS组在出血结束时小肠、肝脏和左肾的MAP和微血管组织灌注均显著减少。复苏后，未观察到组间显著差异。THS诱导与出血期后Cr、AST、CPK、IL-6、IL-10和TNF-α的血浆浓度从基线值显著增加2 - 3倍相关，THS诱导的小鼠表现出显著增加的组织学损伤评分。THS诱导后观察到的MAP和微血管组织灌注的快速下降以及复苏后的逐渐恢复，反映了THS实验小鼠模型的成功建立。
This study established an experimental mouse model of trauma-hemorrhagic shock (THS). THS-induced mice (C57BL/6J, n=33) were subjected to femoral fracture, ischemia for 90 min, and resuscitation for 15 min. The sham-operated mice (C57BL/6J, n=33) underwent the same anesthetic and surgical procedures, but neither trauma-hemorrhage nor fluid resuscitation were performed. Mean arterial pressure (MAP) and microvascular tissue perfusion over the small intestine, liver, and left kidney were longitudinally measured in all mice. Blood was collected for analysis at baseline and 3, 6, 12, and 24 h post resuscitation, and the small intestine, liver, and left kidney were resected for hematoxylin and eosin staining 24 h post resuscitation. Compared with the sham group, MAP and microvascular tissue perfusion over the small intestine, liver, and left kidney were all significantly reduced in the THS group at the end of hemorrhage. Following resuscitation, no significant differences were observed between the groups. THS induction was associated with significantly increased plasma concentrations of Cr, AST, CPK, IL-6, IL-10, and TNF-alpha from the baseline values by two- to three-fold after the hemorrhage phase, and THS-induced mice demonstrated significantly increased histological injury scores. The rapid drop in MAP and microvascular tissue perfusion observed following THS induction, and the gradual recovery post resuscitation, reflects the successful establishment of a THS experimental mouse model.