Novel in-growth core system enables functional studies of grassland mycorrhizal mycelial networks
Novel in-growth core system enables functional studies of grassland mycorrhizal mycelial networks
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DOI：
10.1046/j.0028-646x.2001.00273.x
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发表时间：
2001-12-01
期刊：
影响因子：
9.4
通讯作者：
Read, DJ
中科院分区：
文献类型：
--
作者：
Johnson, D;Leake, JR;Read, DJ
描述和测试了一种新的丛枝菌根(AM)菌根菌丝在土壤中的天然群落功能研究的新型生长核心系统。该核心系统的窗口覆盖有35微米孔径的尼龙网，可以防止根部的内生长，但允许AM菌丝穿透。它们被插入草地草皮中，里面要么是消毒过的沙子和一株白三叶‘诱饵’幼苗，要么是没有诱饵植物的未灭菌的自然土壤。研究了通过周期性旋转部分菌核实现的菌丝分离对诱饵植物AM定殖率(实验1)和P-33从土壤向核心外植物的转移(实验2)的影响。菌丝进入(PO4)-P-33标记的菌丝核心的植物的地上部P-33浓度是没有菌丝接触的植物的10倍。结论：这种新的方法使菌根菌丝网络的功能能够在接近自然发生的条件下被评估。(C)新植物学家(2001年)。
A novel in-growth core system, enabling functional studies of natural communities of arbuscular mycorrhizal (AM) mycelia in soil is described and tested.The cores have windows covered with nylon mesh of 35 mum pore size that prevent in-growth of roots but permit penetration of AM hyphae. They were inserted into grassland turf and contained either sterilized sand and a 'bait' seedling of Trifolium repens or nonsterile natural soil without bait plants. The impacts of hyphal severance, achieved by periodic rotation of some of the cores, upon AM colonization of bait plants (experiment 1) and transfer of P-33 from soil to plants outside the cores (experiment 2) were examined.Severance of AM hyphae reduced both AM colonization of bait plants and their shoot P concentrations. The shoot P-33 concentrations of plants with mycelial access to (PO4)-P-33-labelled cores were 10-fold greater than those which had no mycelial access.It is concluded that this novel approach enables the functioning of mycorrhizal mycelial networks to be evaluated under conditions closely simulating those occurring in nature. (C) New Phytologist (2001).