Transcription co-activator Arabidopsis ANGUSTIFOLIA3 (AN3) regulates water-use efficiency and drought tolerance by modulating stomatal density and improving root architecture by the transrepression of YODA (YDA)
Transcription co-activator Arabidopsis ANGUSTIFOLIA3 (AN3) regulates water-use efficiency and drought tolerance by modulating stomatal density and improving root architecture by the transrepression of YODA (YDA)
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DOI：
10.1111/pbi.12324
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发表时间：
2015-09-01
影响因子：
13.8
通讯作者：
Yao, Shun-Qiao
中科院分区：
文献类型：
--
作者：
Meng, Lai-Sheng;Yao, Shun-Qiao
现代农业的目标之一是提高植物的耐旱性和水分利用效率（WUE）。虽然气孔密度与水分利用效率有关，但这种关系的分子机制和工程改造尚未完全清楚。此外，MAPKK激酶基因YODA（YDA）负调控气孔发育。BR-不敏感2与磷酸化相互作用并抑制YDA。然而，YDA是否在转录水平上被调节仍不清楚。缺乏ANGUSTIFOLIA 3（AN 3）活性的植物具有高的干旱胁迫耐受性，因为气孔密度低和改善的根构型。这些植物还表现出增强的WUE通过减少蒸腾而没有明显减少生物量积累。通过靶基因分析，AN 3在转录水平负调控YDA表达。染色质免疫沉淀分析表明，AN 3与YDA启动子的区域在体内。YDA突变体显著降低了an 3突变体的气孔密度和根长，从而证明YDA参与了an 3的耐旱性和WUE的提高。这些组分形成AN 3-YDA复合物，其允许水分亏缺胁迫信号传导整合到气孔的产生或间隔和细胞增殖中，从而导致耐旱性和增强的WUE。
One goal of modern agriculture is the improvement of plant drought tolerance and water-use efficiency (WUE). Although stomatal density has been linked to WUE, the causal molecular mechanisms and engineered alternations of this relationship are not yet fully understood. Moreover, YODA (YDA), which is a MAPKK kinase gene, negatively regulates stomatal development. BR-INSENSITIVE 2 interacts with phosphorylates and inhibits YDA. However, whether YDA is modulated in the transcriptional level is still unclear. Plants lacking ANGUSTIFOLIA3 (AN3) activity have high drought stress tolerance because of low stomatal densities and improved root architecture. Such plants also exhibit enhanced WUE through declining transpiration without a demonstrable reduction in biomass accumulation. AN3 negatively regulated YDA expression at the transcriptional level by target-gene analysis. Chromatin immunoprecipitation analysis indicated that AN3 was associated with a region of the YDA promoter in vivo. YDA mutation significantly decreased the stomatal density and root length of an3 mutant, thus proving the participation of YDA in an3 drought tolerance and WUE enhancement. These components form an AN3-YDA complex, which allows the integration of water deficit stress signalling into the production or spacing of stomata and cell proliferation, thus leading to drought tolerance and enhanced WUE.