Improving the stability of cellulase by immobilization on modified polyvinyl alcohol coated chitosan beads
Improving the stability of cellulase by immobilization on modified polyvinyl alcohol coated chitosan beads
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DOI：
10.1016/j.molcatb.2006.10.005
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发表时间：
2007-04-01
影响因子：
--
通讯作者：
Telefoncu, Azmi
中科院分区：
文献类型：
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作者：
Dincer, Ayse;Telefoncu, Azmi
纤维素酶在各个行业的应用需要高度稳定的酶，能够在极端的 pH 值和温度下发挥作用。本研究的目的是提高酸性纤维素酶在中性 pH 范围内的稳定性。为此，计划用马来酸酐对聚乙烯醇 (PVA) 进行改性，这与许多对酶进行改性的研究形成鲜明对比。将壳聚糖珠涂有聚阴离子改性PVA，并将纤维素酶固定在改性PVA涂覆的壳聚糖珠上。由于这种修改，酶的最适 pH 值从 pH 4.0 变为 7.0，并且固定化纤维素酶珠在中性 pH 范围内表现出比游离酶更好的 pH 稳定性。固定化纤维素酶的活性产率为87%，固定化后最适温度没有变化。 (c) 2006 Elsevier B.V. 保留所有权利。
The application of cellulases in various industries demands highly stable enzymes, able to perform at extreme pH values and temperatures. In this study, improving the stability of the acid cellulase in the neutral pH range was aimed. For this purpose, modification planned to be done on polyvinyl alcohol (PVA) by maleic anhydride in contrast to many studies in which enzyme was modified. The chitosan beads were coated with polyanionic modified PVA and cellulase was immobilized on the modified PVA coated chitosan beads. As a result of this modification, the pH optimum of enzyme shifted from pH 4.0 to 7.0 and the immobilized cellulase beads showed better pH stability than free enzyme at neutral pH range. The activity yield of the immobilized cellulase was found to be as 87%, and it was found no change of the optimum temperature after immobilization. (c) 2006 Elsevier B.V. All rights reserved.