Impact of oxygen limitation on glycerol-based biopolymer production by bacterial enrichments.
Impact of oxygen limitation on glycerol-based biopolymer production by bacterial enrichments.
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DOI：
10.1016/j.watres.2012.11.039
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发表时间：
2013-03
期刊：
影响因子：
12.8
通讯作者：
Helena Moralejo-Gárate;R. Kleerebezem;A. Mosquera-Corral;M. V. van Loosdrecht
中科院分区：
文献类型：
--
作者：
Helena Moralejo-Gárate;R. Kleerebezem;A. Mosquera-Corral;M. V. van Loosdrecht
生物乙醇和生物柴油产量的增加导致了大量粗甘油的产生，导致需要对这些废液进行有效的有价化。其中一个价态选择可以是通过在盛宴-饥荒过程中使用微生物群落工程将粗甘油转化为生物聚合物。在以前的工作中，甘油生产生物聚合物的一个复杂因素是可以形成两种不同类型的聚合物：聚羟基烷酸(PHA)和聚葡萄糖。在这里，我们描述了限制供氧速率对聚合物分布的影响，目的是确定有利于甘油在单一聚合物中转化的条件。生物大分子最大化阶段供氧速率的降低并不影响甘油在PHA和多聚葡萄糖之间的分配，但群落富集阶段的氧气限制有利于多糖的储存。
The increasing production of bioethanol and biodiesel has resulted in the generation of a massive amount of crude glycerol, inducing the need for effective valorization of these waste streams. One of the valorization options could be through conversion of crude glycerol into a biopolymer using microbial community engineering in a feast–famine process. A complicating factor in the production of biopolymers from glycerol encountered in previous works is that two different types of polymers can be formed; polyhydroxyalkanoate (PHA) and polyglucose. Here we describe the effect of limiting the oxygen supply rate on the polymer distribution with the aim of defining the conditions that favour the conversion of glycerol in one single polymer. The decrease of oxygen supply rate during the biopolymer maximization step did not influence glycerol partitioning among PHA and polyglucose, but oxygen limitation during the community enrichment step favoured polyglucose storage over PHA.