THE ORIGIN RECOGNITION COMPLEX HAS ESSENTIAL FUNCTIONS IN TRANSCRIPTIONAL SILENCING AND CHROMOSOMAL REPLICATION
THE ORIGIN RECOGNITION COMPLEX HAS ESSENTIAL FUNCTIONS IN TRANSCRIPTIONAL SILENCING AND CHROMOSOMAL REPLICATION
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DOI：
10.1101/gad.9.8.911
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发表时间：
1995-04-15
影响因子：
10.5
通讯作者：
RINE, J
中科院分区：
文献类型：
--
作者：
FOX, CA;LOO, S;RINE, J
研究了起点识别复合物（ORC）在复制起始和沉默中的作用。ORC基因中的温度敏感性突变导致染色体复制起点处的复制起始缺陷，如通过二维（2-D）原点作图凝胶、叉迁移分析和质粒复制研究所测量。这些数据与ORC作为真核复制起始子的功能一致。一些起源显示更大的复制起始缺陷矿石突变体比别人，揭示起源之间的功能差异。ORC 5的等位基因被分离出来，它们在沉默方面有缺陷，但在复制方面没有缺陷，这表明ORC在沉默中的作用可以与其在复制中的作用分开。在温度敏感的矿石突变体有丝分裂中被捕，温度变化实验引起沉默的损失，表明ORC的细胞周期的S期以外的功能，ORC是需要的沉默状态的维持。
The role of the origin recognition complex (ORC) was investigated in replication initiation and in silencing. Temperature-sensitive mutations in ORC genes caused defects in replication initiation at chromosomal origins of replication, as measured by two-dimensional (2-D) origin-mapping gels, fork migration analysis, and plasmid replication studies. These data were consistent with ORC functioning as a eukaryotic replication initiator. Some origins displayed greater replication initiation deficiencies in ore mutants than did others, revealing functional differences between origins. Alleles of ORC5 were isolated that were defective for silencing but not replication, indicating that ORC's role in silencing could be separated from its role in replication. In temperature-sensitive ore mutants arrested in mitosis, temperature-shift experiments caused a loss of silencing, indicating both that ORC had functions outside of the S phase of the cell cycle and that ORC was required for the maintenance of the silenced state.