Clinical application of breath biomarkers of oxidative stress status
Clinical application of breath biomarkers of oxidative stress status
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DOI：
10.1016/s0891-5849(99)00212-9
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发表时间：
1999-12-01
影响因子：
7.4
通讯作者：
Sehnert, SS
中科院分区：
文献类型：
--
作者：
Risby, TH;Sehnert, SS
分离和量化指示临床状态相关变化的挥发性呼气生物标记物需要开发新技术和应用现有分析化学方法。成功应用这类样本的最大障碍是减少了允许重复分析的样本量(这是所有临床研究的绝对要求)，将感兴趣的分析物(S)从背景分子、水蒸气和其他具有类似物理性质的分子中分离出来，在分析中引入自动化，使用选择性检测系统(电子碰撞质谱仪、火焰光度计、热离子探测器)，以及从人体对象自动采集样本。吸附技术和微量气体分析的进步使这一临床化学领域取得了快速发展。(C)1999年爱思唯尔科学公司。
Isolation and quantification of volatile breath biomarkers indicative of relevant alterations in clinical status has required development of new techniques and applications of existing analytical chemical methods. The most significant obstacles to successful application of this type of sample have been reduction in required sample volume permitting replicate analysis (an absolute requirement for all clinical studies), separation of the analyte(s) of interest from background molecules, water vapor and other molecules with similar physical properties, introduction of automation in analysis and the use of selective detection systems (electron impact mass spectrometry, flame photometric, thermionic detectors), and automated sample collection from the human subject. Advances in adsorption technology and trace gas analysis have permitted rapid progress in this area of clinical chemistry. (C) 1999 Elsevier Science Inc.