In Vivo Electromechanical Reshaping of Ear Cartilage in a Rabbit Model A Minimally Invasive Approach for Otoplasty
In Vivo Electromechanical Reshaping of Ear Cartilage in a Rabbit Model A Minimally Invasive Approach for Otoplasty
复制标题
DOI：
10.1001/2013.jamafacial.2
复制
发表时间：
2013-01-01
影响因子：
--
通讯作者：
Wong, Brian J. F.
中科院分区：
文献类型：
--
作者：
Oliaei, Sepehr;Manuel, Cyrus;Wong, Brian J. F.
目的：报告第一次成功的研究，以日期在体内的耳软骨的机电重塑在兔model.Methods：新西兰白色兔耳重塑经皮针电极机电重塑（5 V，4分钟），然后支撑4周。治疗10只耳朵，其中2只接受假手术并作为对照。使用铂电极阵列进行治疗，该铂电极阵列由穿过耳朵中的弯曲区域插入的4排平行针组成。4周后处死动物，取耳片进行常规光镜和共聚焦显微镜（活-死荧光分析）观察。结果：所有处理的耳均出现明显的形态改变（平均102.4度，范围87度~ 122度）。对照耳表现出最小的形状保持（平均值，14.5度;范围，4度-25度）。表皮和附件结构在重塑耳中得以保留，并且在所有标本中均观察到新软骨形成。共聚焦显微镜显示局部区域的无活力软骨细胞（
Objective: To report the first successful study to date of in vivo electromechanical reshaping of ear cartilage in a rabbit model.Methods: Ears of New Zealand white rabbits were reshaped using percutaneous needle electrode electromechanical reshaping (5 V for 4 minutes) and were then bolstered for 4 weeks. Ten ears were treated, with 2 undergoing sham procedures and serving as controls. The treatment was performed using a platinum array of electrodes consisting of 4 parallel rows of needles inserted across the region of flexures in the ear. After 4 weeks, the animals were killed, and the ears were photographed and sectioned for conventional light microscopy and confocal microscopy (live-dead fluorescent assays).Results: Significant shape change was noted in all the treated ears (mean, 102.4 degrees; range, 87 degrees-122 degrees). Control ears showed minimal shape retention (mean, 14.5 degrees; range, 4 degrees-25 degrees). Epidermis and adnexal structures were preserved in reshaped ears, and neochondrogenesis was noted in all the specimens. Confocal microscopy demonstrated a localized zone of nonviable chondrocytes (