Highly sensitive multiplexed DNA detection using multi-walled carbon nanotube-based multicolor nanobeacon
Highly sensitive multiplexed DNA detection using multi-walled carbon nanotube-based multicolor nanobeacon
复制标题
使用基于多壁碳纳米管的多色纳米信标进行高灵敏度多重 DNA 检测
DOI：
10.1016/j.talanta.2013.02.003
复制
发表时间：
2013-05-15
期刊：
影响因子：
6.1
通讯作者：
Zhao, Yanchun
中科院分区：
文献类型：
--
作者：
Chen, Jia;Huang, Yong;Zhao, Yanchun
研制了一种高灵敏度、高选择性的多壁碳纳米管（MWCNT）荧光纳米信标，用于均相溶液中DNA的多重分析。在这项工作中，三种不同的染料标记的DNA发夹吸附在多壁碳纳米管表面通过π堆积，这使得染料和多壁碳纳米管接近，并导致染料的荧光猝灭。当将目标DNA加入到溶液中时，目标DNA与探针特异性杂交，形成稳定的DNA双链体，这削弱了探针与多壁碳纳米管之间的相互作用，导致染料的荧光恢复。通过使用三个15-mer DNA片段作为原理验证分析物，所提出的方法表现出良好的分析性能。获得的检测限在35-42 pM的范围内。此外，该方法还表现出优异的区分单核苷酸多态性的能力。(C)2013爱思唯尔有限公司版权所有。
A highly sensitive and selective multi-walled carbon nanotube (MWCNT)-based multicolor fluorescent nanobeacon is developed for multiplexed analysis of DNA in homogeneous solution. In this work, three different dye-labeled DNA hairpins were adsorbed on MWCNTs surface via pi-stacking, which brings the dyes and MWCNTs into close proximity and leads to the quenching of fluorescence of the dyes. When target DNAs were added to the solution, the target DNAs specifically hybridize with the probes to form stable DNA duplexes, which weakens the interactions between the probes and MWCNTs, and results in the fluorescence recovery of the dyes. By using three 15-mer DNA fragments as proof-of-principle analytes, the proposed method showed good analytical performance. The limits of detection obtained were in the range of 35-42 pM. Moreover, this method also exhibits an excellent ability to discriminate between single nucleotide polymorphisms. (C) 2013 Elsevier B.V. All rights reserved.