Red Seaweed Enzyme-catalyzed Bromination of Bromophenol Red: An Inquiry-based Kinetics Laboratory Experiment for Undergraduates
Red Seaweed Enzyme-catalyzed Bromination of Bromophenol Red: An Inquiry-based Kinetics Laboratory Experiment for Undergraduates
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DOI：
10.1002/bmb.20256
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发表时间：
2009-03-01
影响因子：
1.4
通讯作者：
Panijpan, Bhinyo
中科院分区：
文献类型：
--
作者：
Jittam, Piyachat;Boonsiri, Patcharee;Panijpan, Bhinyo
卤代过氧化物酶由于其在制药工业和环境净化中日益重要而引起基础和应用生物科学家的兴趣。在生命科学、农业科学和健康科学本科生的引导探究式实验室实验中，来自红色海藻的溴过氧化物酶被用来溴化溴酚红，一种新的起始底物。底物和酶的浓度依赖于这种酶催化的反应如下比色产生的反应，学生与他们的同龄人相比的初始速率。学生们作为合作小组工作，部分设计自己的实验并进行实验。在进行实验室实验时，他们最少受到教员和助教的指导。为了让学生在实验室前参与，进行了涉及酶诱导颜色变化的简短活动。在实验课结束时，学生小组在全班面前讨论他们的结果，并得出自己的结论。大部分学生对酶动力学的重要概念有较好的理解，对以学生为中心的实验练习整体态度良好。
Haloperoxidase enzymes are of interest for basic and applied bioscientists because of their increasing importance in pharmaceutical industry and environmental cleanups. In a guided inquiry-based laboratory experiment for life-science, agricultural science, and health science undergraduates, the bromoperoxidase from a red seaweed was used to brominate bromophenol red, a novel starting substrate. Substrate and enzyme concentration dependence of this enzyme-catalyzed reaction as followed colorimetrically yielded initial rates of reaction that the students compared with those of their peers. The students worked as collaborative groups partially designing their own experiments and carrying them out. In performing the laboratory experiment, they were minimally guided by the instructor and teaching assistants. To engage the students before the laboratory, a short activity involving the enzyme-induced color change was carried out. At the end of the laboratory session, student groups discussed their results in front of the class and reached their own conclusions. Most students had better understanding of important concepts in enzyme kinetics and showed good attitude toward the overall student-centered laboratory exercise.