Prostatic secretory protein (PSP94) expression in human female reproductive tissues, breast and in endometrial cancer cell lines
Prostatic secretory protein (PSP94) expression in human female reproductive tissues, breast and in endometrial cancer cell lines
复制标题
DOI：
10.1677/joe.0.1650425
复制
发表时间：
2000-05-01
影响因子：
4
通讯作者：
Han, VKM
中科院分区：
文献类型：
--
作者：
Baijal-Gupta, M;Clarke, MW;Han, VKM
PSP 94（beta microalbuminoprotein，β MSP）是正常人前列腺分泌的三种主要蛋白质之一。应用逆转录聚合酶链反应（RT-PCR）和Southern杂交技术，检测了人子宫内膜、子宫肌层、卵巢、乳腺、胎盘及人子宫内膜癌细胞系KI-E和AN 3CA中的PSP 94转录产物。结果证实了两个独立的克隆从正常人乳腺组织和其他两个从KLE细胞分别序列分析。其序列与人前列腺PSP 94 cDNA编码区序列完全一致。使用来自子宫内膜组织的RNA，通过RT-PCR扩增两种不同的转录物，其类似于487 bp，相当于前列腺PSP 94和类似于381 bp，对应于前列腺PSP 57，其交替剪接形式。然而，人卵巢癌、乳腺癌、胎盘癌和子宫内膜癌细胞系（KLE、AN 3 CA）仅显示类似于487 bp的全长PSP 94转录本。我们进一步证明了原位杂交，PSP 94 mRNA的表达，特别是在腺上皮细胞，而不是在人子宫内膜和乳腺组织的基质。此外，利用原位杂交数据的图像分析，PSP 94 mRNA在周期性子宫内膜组织和乳腺组织中的表达水平证实了在周期性子宫内膜中的差异表达水平（P
PSP94 (beta microseminoprotein, beta MSP) is one of the three major proteins secreted by the normal human prostate gland. Using reverse transcriptase polymerase chain reaction (RT-PCR) and Southern blotting, PSP94 transcripts were shown in human endometrium, myometrium, ovary, breast, placenta and in the human endometrial cancer cell lines KI-E and AN3 CA, Primers used in these studies were specific for human prostate PSP94, and were derived from its flanking non-coding regions. The results were confirmed by sequence analysis of two independently derived clones from normal human breast tissues and the other two from KLE cells respectively. The sequences were identical with the coding sequence of human prostate PSP94 cDNA. Using RNA from the endometrial tissues, two different transcripts of similar to 487 bp, equivalent to prostate PSP94 and similar to 381 bp, corresponding to prostate PSP57, its alternately spliced form, were amplified by RT-PCR. Human ovary, breast, placenta and endometrial cancer cell lines (KLE, AN3 CA), however, showed only the full length, similar to 487 bp, PSP94 transcript. We further demonstrated by in situ hybridization that PSP94 mRNA is expressed specifically in the glandular epithelial cells, and not in the stroma of both the human endometrial and breast tissues. Further, using image analysis of in situ hybridization data, the levels of PSP94 mRNA in the cycling endometrial tissues and in breast confirmed the differential levels of expression in the cycling endometrium (P