Techniques for long-term multisite neuronal ensemble recordings in behaving animals
Techniques for long-term multisite neuronal ensemble recordings in behaving animals
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DOI：
10.1006/meth.2001.1231
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发表时间：
2001-10-01
期刊：
影响因子：
4.8
通讯作者：
Nicolelis, MAL
中科院分区：
文献类型：
--
作者：
Kralik, JD;Dimitrov, DF;Nicolelis, MAL
我们对神经系统理解的进步将与用于研究这些系统的实验技术的改进齐头并进。本文介绍了一系列的方法学发展，旨在提高所需的方法的权力，同时记录在清醒的，行为动物的大脑的广泛区域的神经元群体。首先，我们的实验室在电极设计方面取得了许多进展，包括可移动束和阵列电极以及更小的电极组件。其次，为了在灵长类动物中进行更长时间和更复杂的多电极植入手术，我们通过采用类似于人类神经麻醉的全面生理监测来修改我们的手术程序。我们还开发了手术植入技术，旨在最大限度地减少脑组织损伤并促进穿透皮质表面。第三，我们将新技术整合到我们的神经集成，刺激和行为记录实验中，以提供更详细的实验变量测量。最后，实验室正在使用新的数据分析技术来分析越来越多的数据。(C)2001年，爱思唯尔科学。
Advances in our understanding of neural systems will go hand in hand with improvements in the experimental techniques used to study these systems. This article describes a series of methodological developments aimed at enhancing the power of the methods needed to record simultaneously from populations of neurons over broad regions of the brain in awake, behaving animals. First, our laboratory has made many advances in electrode design, including movable bundle and array electrodes and smaller electrode assemblies. Second, to perform longer and more complex multielectrode implantation surgeries in primates, we have modified our surgical procedures by employing comprehensive physiological monitoring akin to human neuroanesthesia. We have also developed surgical implantation techniques aimed at minimizing brain tissue damage and facilitating penetration of the cortical surface. Third, we have integrated new technologies into our neural ensemble, stimulus and behavioral recording experiments to provide more detailed measurements of experimental variables. Finally, new data analytical techniques are being used in the laboratory to analyze increasingly large quantities of data. (C) 2001 Elsevier Science.