Antimicrobial Activity of Chitosan-Based Films Enriched with Green Tea Extracts on Murine Norovirus, Escherichia coli, and Listeria innocua
Antimicrobial Activity of Chitosan-Based Films Enriched with Green Tea Extracts on Murine Norovirus, Escherichia coli, and Listeria innocua
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DOI：
10.1155/2020/3941924
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发表时间：
2020-07-04
影响因子：
4
通讯作者：
Pascall, Melvin A.
中科院分区：
文献类型：
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作者：
Amankwaah, Collins;Li, Jianrong;Pascall, Melvin A.
可食用膜可被设计成用作抗微生物剂的载体，并用于控制致病性食源性病毒和细菌。本研究将绿色茶提取物（GTE）溶解于壳聚糖成膜溶液（FFS）中，并将其用于制备干燥的壳聚糖可食性膜。作为对照，还将GTE溶解在去离子水（DW）中。FFS和干燥的壳聚糖膜与GTE和DW没有壳聚糖都进行了评估，对鼠诺如病毒（MNV-1），大肠杆菌K12，和无害李斯特菌。将FFS和含GTE的DW与类似于10（7）PFU/ml的病毒悬液孵育3 h。将具有GTE的壳聚糖膜在23 +/-1 ℃下孵育4和24小时。结果显示，含有1、1.5和2.5%水性GTE的DW在3小时暴露后分别显著（p < 0.05）减少MNV-1噬斑1.7、2.5和3.3个对数。同样，FFS含有2.5%和5.0%的GTE减少MNV-1计数2.5和4.0对数，分别在3小时的曝光后。含有5、10和15%GTE的干燥壳聚糖膜也有效对抗MNV-1感染性。孵育24小时后，5%和10%壳聚糖GTE膜分别产生1.6和4.5 log的显著（p < 0.05）滴度降低。含有15%GTE的壳聚糖膜在24小时内将MNV-1噬菌斑减少到不可检测的水平。所有壳聚糖GTE膜均降低E. coli K12和L.接触24小时后，胰蛋白酶大豆肉汤中的无害种群达到不可检测的水平。这项研究的结果表明，富含GTE的可食用膜具有减少食源性病毒和细菌的潜力。
Edible films can be designed to serve as carriers of antimicrobial agents and be used to control pathogenic foodborne viruses and bacteria. This research tested this concept by dissolving green tea extract (GTE) in chitosan film-forming solutions (FFS) and using it to prepare dried chitosan edible films. As a control, the GTE was also dissolved in deionized water (DW). The FFS and the dried chitosan films with the GTE and the DW without chitosan were all evaluated against murine norovirus (MNV-1), Escherichia coli K12, and Listeria innocua. Both the FFS and the DW with GTE were incubated with similar to 10(7) PFU/ml of the virus suspensions for 3 h. The chitosan films with GTE were incubated for 4 and 24 h at 23 +/- 1 degrees C. The results showed that the DW containing 1, 1.5, and 2.5% aqueous GTE, significantly (p < 0.05) reduced MNV-1 plaques by 1.7, 2.5, and 3.3 logs after 3 h exposure, respectively. Similarly, FFS containing 2.5 and 5.0% GTE reduced MNV-1 counts by 2.5 and 4.0 logs, respectively, after 3 h exposure. The dried chitosan films with 5, 10, and 15% GTE were also effective against MNV-1 infectivity. After 24 h incubation, the 5 and 10% chitosan GTE films produced significant (p < 0.05) titer reductions of 1.6 and 4.5 logs, respectively. Chitosan films containing 15% GTE reduced MNV-1 plaques to undetectable levels in 24 h. All chitosan GTE films reduced E. coli K12 and L. innocua populations to undetectable levels in tryptic soy broth after 24 h exposure. The results of this study showed that edible films enriched with GTE have potential to reduce both foodborne viruses and bacteria.