Response of the Pseudomonas host chromosomal transcriptome to carriage of the IncP-7 plasmid pCAR1
Response of the Pseudomonas host chromosomal transcriptome to carriage of the IncP-7 plasmid pCAR1
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DOI：
10.1111/j.1462-2920.2009.02110.x
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发表时间：
2010-06-01
影响因子：
5.1
通讯作者：
Nojiri, Hideaki
中科院分区：
文献类型：
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作者：
Shintani, Masaki;Takahashi, Yurika;Nojiri, Hideaki
质粒携带需要质粒或宿主染色体上的基因通过协同转录调节进行适当表达。为了阐明质粒携带对宿主染色体的影响，我们使用不相容组P-7原型质粒pCAR 1（参与咔唑降解）和三种不同的假单胞菌菌株比较了无质粒和含质粒宿主菌株的染色体RNA图谱。拥有pCAR 1改变了与每个宿主中的铁获取系统相关的基因表达。主要铁载体绿脓菌荧光素的表达在含有质粒的恶臭假单胞菌KT 2440和铜绿假单胞菌PAO 1中比在不含质粒的宿主菌株中更高，部分原因是pCAR 1上咔唑降解基因的表达。pCAR 1的携带特异性地上调了恶臭假单胞菌KT 2440中编码耐药-增殖-细胞分裂家族的外排泵的mexEFoprN操纵子，而其他物种中的正向同源基因的表达保持不变。与不含pCAR 1的KT 2440相比，mexEFoprN基因的诱导增加了含pCAR 1的KT 2440对氯霉素的耐药性。我们的研究结果表明，拥有pCAR 1通过表达pCAR 1和宿主染色体上的基因改变宿主的生长速率。
P>Plasmid carriage requires appropriate expression of the genes on the plasmid or host chromosome through cooperative transcriptional regulation. To clarify the impact of plasmid carriage on the host chromosome, we compared the chromosomal RNA maps of plasmid-free and plasmid-containing host strains using the incompatibility group P-7 archetype plasmid pCAR1, which is involved in carbazole degradation, and three distinct Pseudomonas strains. The possession of pCAR1 altered gene expression related to the iron acquisition systems in each host. Expression of the major siderophore pyoverdine was greater in plasmid-containing P. putida KT2440 and P. aeruginosa PAO1 than in the plasmid-free host strains, in part due to the expression of carbazole-degradative genes on pCAR1. The mexEFoprN operon encoding an efflux pump of the resistance-nodulation-cell division family was specifically upregulated by the carriage of pCAR1 in P. putida KT2440, whereas the expression of orthologous genes in the other species remained unaltered. Induction of the mexEFoprN genes increased the resistance of pCAR1-containing KT2440 to chloramphenicol compared with pCAR1-free KT2440. Our findings indicate that the possession of pCAR1 altered the growth rate of the host via the expression of genes on pCAR1 and the host chromosomes.