MiR‐143 enhances adipogenic differentiation of 3T3‐L1 cells through targeting the coding region of mouse pleiotrophin
MiR‐143 enhances adipogenic differentiation of 3T3‐L1 cells through targeting the coding region of mouse pleiotrophin
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DOI：
10.1016/j.febslet.2011.09.015
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发表时间：
2011-10
期刊：
影响因子：
3.5
通讯作者：
Can Yi;Wei Xie;Fu Li;Q. Lv;Jie He;Jiangbin Wu;D. Gu;Naihan Xu;Yaou Zhang
中科院分区：
文献类型：
--
作者：
Can Yi;Wei Xie;Fu Li;Q. Lv;Jie He;Jiangbin Wu;D. Gu;Naihan Xu;Yaou Zhang
前脂肪细胞的成脂分化是一个复杂的过程，受多种因素调控，包括mirna和细胞因子。MiR-143是一种众所周知的促进脂肪形成的miRNA。多营养因子（PTN）是一种肝素结合生长因子，在脂肪形成中起负作用。在这项研究中，我们证明PTN是miR-143在3T3-L1前脂肪细胞成脂分化过程中的靶基因。MiR-143通过与小鼠PTN编码区MiR-143的靶位点相互作用，下调PTN的表达。靶位上游的罕见密码子调控mir143诱导的PTN的翻译敲低，这为脂肪分化的机制提供了更多的见解。
Adipogenic differentiation of preadipocytes is a complex process regulated by various factors including miRNAs and cytokines. MiR-143 is a well known miRNA that enhances adipogenesis. Pleiotrophin (PTN), a heparin-binding growth factor, plays a negative role in adipogenesis. In this investigation, we demonstrate that PTN is a target gene of miR-143 during adipogenic differentiation in 3T3-L1 preadipocytes. MiR-143 down regulates PTN expression through interaction with a target site of miR-143 in the coding region of mouse PTN. The rare codons upstream of the target site regulate miR143-induced translational knockdown of PTN, which provides more insight into the mechanism of adipogenic differentiation.