Immunization of BALB/c mice with Brucella abortus 2308ΔwbkA confers protection against wild-type infection.
Immunization of BALB/c mice with Brucella abortus 2308ΔwbkA confers protection against wild-type infection.
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用流产布鲁氏菌 2308 Delta wbkA 免疫 BALB/c 小鼠可提供针对野生型感染的保护
DOI：
10.4142/jvs.2015.16.4.467
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发表时间：
2015
影响因子：
1.8
通讯作者：
Chen CF
中科院分区：
文献类型：
--
作者：
Li ZQ;Gui D;Sun ZH;Zhang JB;Zhang WZ;Zhang H;Guo F;Chen CF
布鲁氏菌病是一种人畜共患疾病，可引起动物和人类疾病。接种疫苗是预防布鲁氏菌病的主要措施，但目前无法区分接种疫苗的动物和自然感染的动物。因此，本研究构建了布鲁氏菌（B.）2308ΔwbkA突变株的毒力。2308ΔwbkA在小鼠巨噬细胞（RAW 264.7）和BALB/c小鼠中的存活率明显降低，并诱导小鼠产生了较高的保护性免疫。wbkA突变体引起抗布鲁氏菌特异性免疫球蛋白G应答并诱导γ干扰素分泌。在小鼠血清中可以检测到2308ΔwbkA的抗体，这意味着该蛋白作为诊断抗原的潜力。WbkA抗原将允许感染和接种动物之间的血清学区分。这些结果表明，2308ΔwbkA是一种潜在的16 M减毒疫苗。该疫苗将在绵羊中进行进一步评价。
Brucellosis is a zoonotic disease that causes animal and human diseases. Vaccination is a major measure for prevention of brucellosis, but it is currently not possible to distinguish vaccinated animals from those that have been naturally infected. Therefore, in this study, we constructed the Brucella (B.) abortus 2380 wbkA mutant (2308ΔwbkA) and evaluated its virulence. The survival of 2308ΔwbkA was attenuated in murine macrophage (RAW 264.7) and BALB/c mice, and it induced high protective immunity in mice. The wbkA mutant elicited an anti-Brucella-specific immunoglobulin G response and induced the secretion of gamma interferon. Antibodies to 2308ΔwbkA could be detected in sera from mice, implying the potential for use of this protein as a diagnostic antigen. The WbkA antigen would allow serological differentiation between infected and vaccinated animals. These results suggest that 2308ΔwbkA is a potential attenuated vaccine against 16M. This vaccine will be further evaluated in sheep.