Cytochrome b gene structure and consequences tor resistance to Qo inhibitor fungicides in plant pathogens
Cytochrome b gene structure and consequences tor resistance to Qo inhibitor fungicides in plant pathogens
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DOI：
10.1002/ps.1236
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发表时间：
2006-06-01
影响因子：
4.1
通讯作者：
Gisi, Ulrich
中科院分区：
文献类型：
--
作者：
Grasso, Valeria;Palermo, Simona;Gisi, Ulrich
对不同农艺学上重要的植物病原体的细胞色素B（cyt B）基因结构进行了表征，所述不同农艺学上重要的植物病原体例如小麦隐匿柄锈菌（Puccinia recondita f sp triangulae（Erikss）CO约翰斯顿、小麦隐匿柄锈菌（P.graminis f sp triangulae Erikss and Hennings）、小麦条锈菌（P.striiformis f sp triangulae Erikss）、燕麦冠锈菌（P.coronata f sp avenae P Syd & Syd）、大麦隐匿柄锈菌（P.hordei GH Otth）、黑麦隐匿柄锈菌（P.secalis Robbridata）、高粱隐匿柄锈菌（P.sorghi Schwein）、大麦隐匿柄锈菌（P.horiana Henn）、顶生单胞锈菌（Uromyces apaculatus（Pers）Unger）、豆薯层锈菌（Phakopsora pachyrhizi Syd & P Syd）、马铃薯叶斑病菌（Hemileia vastatrix Berk & Broome）、番茄早疫病（Alternaria solani Sorauer）、烟草赤星病菌（A alternata（Fr）Keissl）和葡萄生单轴霉（Plasmopara viticola（Berk & Curt）Berlese & de Toni）。测序片段包括两个热点区域，其中可能发生赋予对QoI杀真菌剂抗性的突变。将这些病原体的cyt B基因结构与公共数据库中的其他物种进行比较，包括产嗜球果伞素真菌Mycena galopoda（Pers）P Kumm、酿酒酵母Meyer ex汉森、苹果黑星病菌Venturia inaequalis（Cooke）Winter和斐济球腔菌Mycosphaerella fijiensis Morelet。在所有的锈菌物种中，以及在茄病立枯丝核菌中，由突变G143A引起的对QoI杀真菌剂的抗性从未报道过。在这些物种中，在位置143处的甘氨酸密码子后直接观察到I型内含子。该内含子在病原体中不存在，例如A alternata，Blumeria graminis（DC）Speer，Pyricularia grisea Sacc，Mycosphaerella graminicola（Fuckel）J Schrot，M fijiensis，V inaequalis和P viticola，其中已经发生对QoI杀真菌剂的抗性，并且在抗性基因型的位置143处甘氨酸被丙氨酸取代。本作者预测，密码子143中的核苷酸取代将阻止内含子的剪接，导致细胞色素B缺陷，这是致命的。因此，对基于G143A的QoI杀真菌剂的抗性的进化不太可能在携带直接在该密码子之后的内含子的病原体中进化。(c)2006年化学工业学会。
The cytochrome b (cyt b) gene structure was characterized for different agronomically important plant pathogens, such as Puccinia recondita f sp tritici (Erikss) CO Johnston, P graminis f sp tritici Erikss and Hennings, P striiformis f sp tritici Erikss, P coronata f sp avenae P Syd & Syd, P hordei GH Otth, P recondita f sp secalis Roberge, P sorghi Schwein, P horiana Henn, Uromyces appendiculatus (Pers) Unger, Phakopsora pachyrhizi Syd & P Syd, Hemileia vastatrix Berk & Broome, Alternaria solani Sorauer, A alternata (Fr) Keissl and Plasmopara viticola (Berk & Curt) Berlese & de Toni. The sequenced fragment included the two hot spot regions in which mutations conferring resistance to QoI fungicides may occur. The cyt b gene structure of these pathogens was compared with that of other species from public databases, including the strobilurin-producing fungus Mycena galopoda (Pers) P Kumm, Saccharomyces cerevisiae Meyer ex Hansen, Venturia inaequalis (Cooke) Winter and Mycosphaerella fijiensis Morelet. In all rust species, as well as in A solani, resistance to QoI fungicides caused by the mutation G143A has never been reported. A type I intron was observed directly after the codon for glycine at position 143 in these species. This intron was absent in pathogens such as A alternata, Blumeria graminis (DC) Speer, Pyricularia grisea Sacc, Mycosphaerella graminicola (Fuckel) J Schrot, M fijiensis, V inaequalis and P viticola, in which resistance to QoI fungicides has occurred and the glycine is replaced by alanine at position 143 in the resistant genotype. The present authors predict that a nucleotide substitution in codon 143 would prevent splicing of the intron, leading to a deficient cytochrome b, which is lethal. As a consequence, the evolution of resistance to QoI fungicides based on G143A is not likely to evolve in pathogens carrying an intron directly after this codon. (c) 2006 Society of Chemical Industry.