The application of transcription activator-like effector nucleases for genome editing in C-elegans
The application of transcription activator-like effector nucleases for genome editing in C-elegans
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DOI：
10.1016/j.ymeth.2014.04.013
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发表时间：
2014-08-01
期刊：
影响因子：
4.8
通讯作者：
Ou, Guangshuo
中科院分区：
文献类型：
--
作者：
Yi, Peishan;Li, Wei;Ou, Guangshuo
The nematode Caenorhabditis elegans has been a powerful model system for biomedical research in the past decades, however, the efficient genetic tools are still demanding for gene knockout, knock-in or conditional gene mutations. Transcription activator-like effector nucleases (TALENs) that comprise a sequence-specific DNA-binding domain fused to a Fokl nuclease domain facilitate the targeted genome editing in various cell types or organisms. Here we summarize the recent progresses and protocols using TALENs in C elegans that generate gene mutations and knock-ins in the germ line and the conditional gene knockout in somatic tissues. (C) 2014 Elsevier Inc. All rights reserved.
