Morphological and glycan features of the camel oviduct epithelium
Morphological and glycan features of the camel oviduct epithelium
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DOI：
10.1016/j.aanat.2014.02.007
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发表时间：
2014-01-01
影响因子：
2.2
通讯作者：
Desantis, Salvatore
中科院分区：
文献类型：
--
作者：
Accogli, Gianluca;Monaco, Davide;Desantis, Salvatore
用形态计量学、扫描电子显微镜和糖组织化学方法研究了单峰骆驼卵泡波生长期(GP)和成熟期(MP)输卵管的区域差异。壶腹上皮高度显着增加，峡部从GP到MP的高度显着降低。扫描电子显微镜下，无纤毛细胞可见顶端气泡(分泌型)或短微绒毛。糖萼纤毛在GP和MP的输卵管中表达以唾液酸连接的α2，6与Gal/GalNAc结合的糖链(SNA亲和力)，在MP的输卵管中表达以唾液酸连接的α2，3-Galβ1，3GalNAc(MAL II和KOH-唾液酸酶(K-S)-PNA染色)。非纤毛细胞从核上胞浆到管腔表面均有凝集素结合部位。壶腹非纤毛细胞在GP和MP期间呈O-连接(粘液型)唾液多糖(MAL II和K-S-PNA)，在MP期间呈N-连接唾液多糖(SNA)。峡部非纤毛细胞在GP和MP中表达SNA活性，在MP中表达K-S-PNA结合体，在GP中表达MAL II和PNA亲和力(Galβ1，3GalNAc)。Galβ1，3GalNAc在GP UTJ的非纤毛细胞中涎化。GP和MP的管腔表面缺乏半乳糖β1，3GalNAc，但表达α2，6和α2，3连接的唾液酸。在GP，腔内物质在壶腹与SNA、MAL II、K-S-PNA反应，在峡部和UTJ仅与MAL II反应。这些结果表明，骆驼输卵管上皮的形态和糖链模式在卵泡波动过程中发生了变化，这可能与区域特有的功能有关。(C)2014年爱思唯尔股份有限公司。版权所有。
This study describes regional differences in the oviduct of the one-humped camel (Camelus dromedarius) during the growth phase (GP) and the mature phase (MP) of the follicular wave by means of morphometry, scanning electron microscopy (SEM) and glycohistochemistry investigations. Epithelium height significantly increased in the ampulla and decreased in the isthmus passing from the GP to the MP. Under SEM, non-ciliated cells displayed apical blebs (secretory) or short microvilli. Cilia glycocalyx expressed glycans terminating with sialic acid linked alpha 2,6 to Gal/GalNAc (SNA affinity) throughout the oviducts of GP and MP and sialic acid linked alpha 2,3 to Gal beta 1,3GalNAc (MAL II and KOH-sialidase (K-s)-PNA staining) throughout the MP oviducts. Non-ciliated cells displayed lectin-binding sites from the supra-nuclear cytoplasm to the luminal surface. Ampulla non-ciliated cells showed O-linked (mucin-type) sialoglycans (MAL II and K-s-PNA) during GP and MP and N-linked sialoglycans (SNA) during the MP. Isthmus non-ciliated cells expressed SNA reactivity in GP and MP, also K-s-PNA binders in MP, and MAL II and PNA affinity (Gal beta 1,3GalNAc) during GP. Gal beta 1,3GalNAc was sialilated in the non-ciliated cells of GP UTJ. Luminal surface lacked of Gal beta 1,3GalNAc in GP and MP, whereas it expressed alpha 2,6- and alpha 2,3-linked sialic acids. In GP intraluminal substance reacted with SNA, MAL II, K-s-PNA in ampulla and only with MAL II in the isthmus and UTJ. These results demonstrate that the morphology and the glycan pattern of the camel oviductal epithelium vary during the follicular wave and that could relate to the region-specific functions. (C) 2014 Elsevier GmbH. All rights reserved.