Specific cytolysis of fresh tumor cells by an autologous killer T cell line derived from an adult T cell leukemia/lymphoma patient.
Specific cytolysis of fresh tumor cells by an autologous killer T cell line derived from an adult T cell leukemia/lymphoma patient.
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来自成人 T 细胞白血病/淋巴瘤患者的自体杀伤 T 细胞系对新鲜肿瘤细胞的特异性细胞溶解。
DOI：
10.4049/jimmunol.133.2.1037
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发表时间：
1984
影响因子：
4.4
通讯作者：
Y. Hinuma
中科院分区：
文献类型：
--
作者：
M. Kannagi;K. Sugamura;K. Kinoshita;H. Uchino;Y. Hinuma
1例成人T细胞白血病/淋巴瘤（ATL）患者的细胞毒性T细胞（Tc）体外杀伤新鲜自体淋巴瘤细胞。本研究采用自体ATLV细胞系（ILT），在白细胞介素2（IL 2）存在下，经多次体外刺激，从缓解期患者外周血白细胞（PBL）中诱导产生Tc。以相同的方式刺激来自其他八名ATL患者的PBL，并且来自缓解期患者的应答细胞也显示出对携带ATL病毒（ATLV）的细胞特异性的细胞毒性。在复发时获得新鲜淋巴瘤细胞，并用作自体Tc诱导的靶细胞。他们成为敏感的Tc在体外孵育4小时内，其敏感性增加，孵育时间至少为12小时。ATLV抗原对这些淋巴瘤细胞的细胞表面上，然而，未检测到放射免疫测定在这些孵育期间，但可检测到孵育16小时后。此外，针对淋巴瘤细胞的细胞毒性被用作“冷”靶竞争细胞的自体ILT细胞完全抑制。这些结果表明，Tc的靶抗原在自体ILT细胞和淋巴瘤细胞上都有表达，并且可能与血清学方法检测到的ATLV抗原不同。此外，数据表明同种异体限制的Tc，优先杀死同种异体ATLV轴承细胞共享几个HLA抗原。
Cytotoxic T cells (Tc) derived from one patient with adult T cell leukemia/lymphoma (ATL) killed fresh autologous lymphoma cells in vitro. The Tc were induced from peripheral blood leukocytes (PBL) of this patient during remission by multiple in vitro stimulations with an autologous ATLV-bearing cell line (ILT) that was previously established by cloning of PBL in the presence of interleukin 2 (IL 2). PBL from eight other ATL patients were stimulated in the same manner, and responder cells from a patient in remission also showed cytotoxicity specific for ATL virus (ATLV)-bearing cells. Fresh lymphoma cells were obtained in relapse and were used as target cells for the autologous Tc induced. They became susceptible to the Tc within 4 hr of in vitro incubation, and their susceptibility increased with incubation time for at least 12 hr. ATLV antigens on the cell surface of these lymphoma cells, however, were not detected by radioimmunoassay during these incubation periods, but were detectable after 16 hr of incubation. In addition, cytotoxicity against lymphoma cells was completely inhibited by autologous ILT cells used as "cold" target competitor cells. These findings indicate that the target antigen of the Tc was expressed on both autologous ILT cells and lymphoma cells, and it may be different from ATLV antigens detected by serologic methods. In addition, the data suggested allogeneic restriction of the Tc in that the preferentially killed allogeneic ATLV-bearing cells share several HLA antigens.