A HIGHLY EFFICIENT AND LARGE-SCALE SYNTHESIS OF (2S,3S)-[2,3-2H2]ASPARTIC AND (2S,3R)-[3-2H]ASPARTIC ACIDS VIA AN IMMOBILIZED ASPARTASE-CONTAINING MICROBIAL CELL SYSTEM
A HIGHLY EFFICIENT AND LARGE-SCALE SYNTHESIS OF (2S,3S)-[2,3-2H2]ASPARTIC AND (2S,3R)-[3-2H]ASPARTIC ACIDS VIA AN IMMOBILIZED ASPARTASE-CONTAINING MICROBIAL CELL SYSTEM
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DOI：
10.1021/jo00274a042
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发表时间：
1989-06-23
影响因子：
3.6
通讯作者：
WOODARD, RW
中科院分区：
文献类型：
--
作者：
LEE, KM;RAMALINGAM, K;WOODARD, RW
本文描述了一种含有固定化天冬氨酸酶的大肠杆菌系统的制备和应用，该系统用于从适当标记的富马酸以> 95%的分离产率大规模合成（2S，3S）-[2，3 - 2 H 2]-和（2S，3R）-[3- 2 H]-天冬氨酸，在C-3中心具有> 97%的氘掺入，并且在C-2和C-3中心都是光学纯的。
This paper describes the preparation and utilization of an immobilized aspartase-containing Escherichia coli system for the large-scale synthesis of (2S,3S)-[2,3-2H2]- and (2S,3R)-[3-2H]aspartic acids from the appropriately labeled fumaric acids in > 95% isolated yield, with > 97% deuterium incorporation at the C-3 center and optically pure at both the C-2 and C-3 centers.