Taxonomy and regeneration of a newly recorded Polychaete Capitella teleta (Annelida, Capitellidae) in the coastal water of Shandong, China
Taxonomy and regeneration of a newly recorded Polychaete Capitella teleta (Annelida, Capitellidae) in the coastal water of Shandong, China
复制标题
山东近海新记录多毛类Capitella teleta（环节动物门Capitellidae）的分类与再生
DOI：
10.1007/s00343-021-0378-5
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发表时间：
2021
影响因子：
1.6
通讯作者：
Linlin Zhang
中科院分区：
文献类型：
--
作者：
Qian Li;Yongnan Li;Yue Wang;Xuwen Wu;Linlin Zhang
小头虫属（Capitella）的多毛类物种在中国海域分布广泛，然而，人们对小头虫属的分类学了解甚少，50多年来，在中国采集的标本一直被鉴定为头鳃虫（Capitella capitata，法布里丘斯，1780年）。头鳃虫被认为分布于北极和亚北极地区，因此，在中国海域记录到的头鳃虫很可能是其他物种。基于从山东省东北沿海水域采集的样本开展的一项分类学研究，发现了一个不同的物种——特氏小头虫（Capitella teleta，布莱克等人，2009年），这是该物种首次在中国海域被记录。从形态学上看，特氏小头虫在第8和第9刚节上无腹须毛状刚毛，据此可轻易将其与头鳃虫区分开来。基于线粒体细胞色素c氧化酶亚基I（COI）基因进行的遗传距离和系统发育分析，进一步证实了特氏小头虫的物种身份。此外，通过整装免疫组织化学和化学染色研究了特氏小头虫的再生特性。截肢后，特氏小头虫的伤口在24小时内愈合，截肢3天后（dpa）形成明显的再生芽基。到截肢5天时，肌肉组织开始再生，神经纤维也开始延伸。到截肢7天时，神经突和肌肉组织均显著再生。值得注意的是，到截肢16天时，再生出了十多个体节。作为一种高度机会主义的物种，特氏小头虫分布于中国、日本、韩国、北美洲和地中海地区。有望成为研究发育遗传学和再生进化的优秀模式生物。
The polychaete species of Capitella are widely distributed in the China seas, however little is known about Capitella taxonomy, and specimens collected from China have been identified as Capitella capitata (Fabricius, 1780) for more than 50 years. C. capitata was considered to be Arctic and subarctic in distribution, therefore the records of C. capitata in the China seas probably represent other species. A taxonomic study based on the samples collected from the northeast coastal water of Shandong Province reveals a different species, Capitella teleta Blake et al., 2009, which is recorded in the China seas for the first time. Morphologically, C. teleta can be easily distinguished from C. capitata by the absence of neuropodial capillaries on chaetigers 8 and 9. The identity of C. teleta is further supported by genetic distance and phylogenetic analyses assessed from mitochondrial cytochrome c oxidase subunit I (COI) gene. In addition, the regeneration feature of C. teleta was studied through whole mount immunohistochemistry and chemical staining. After amputation, the wound of C. teleta was healed within 24 h, forming a significant regeneration blastema by 3 days post amputation (dpa). By 5 dpa, muscle tissues regenerated, nerve fibers also extended. By 7 dpa, neurites and muscle tissues are both significantly regenerated. Notably, there are more than ten segments regenerated until 16 dpa. As a highly opportunistic species, Capitella teleta is distributed in China, Japan, Korea, North America, and the Mediterranean. It is expected to be an excellent model for studying developmental genetics and evolution of regeneration.