Self-Healing Conductive Injectable Hydrogels with Antibacterial Activity as Cell Delivery Carrier for Cardiac Cell Therapy
Self-Healing Conductive Injectable Hydrogels with Antibacterial Activity as Cell Delivery Carrier for Cardiac Cell Therapy
复制标题
具有抗菌活性的自愈导电注射水凝胶作为心脏细胞治疗的细胞递送载体
DOI：
10.1021/acsami.6b04911
复制
发表时间：
2016-07-13
影响因子：
9.5
通讯作者：
Ma, Peter X.
中科院分区：
文献类型：
--
作者：
Dong, Ruonan;Zhao, Xin;Ma, Peter X.
细胞治疗是一种很有前途的策略，以再生心肌梗死的心脏组织。具有导电性和自我修复能力的可注射水凝胶作为心脏再生的细胞递送载体是非常理想的。在这里，我们开发了自愈合的导电可注射水凝胶的基础上壳聚糖接枝苯胺四聚体（CS-AT）和二苯并咪唑封端的聚（乙二醇）（PEG-DA）作为心肌梗死的细胞运载工具。在生理条件下，将CS-AT和PEG-DA溶液混合后得到自愈合的电活性水凝胶。用流变仪研究了其快速自愈合行为。研究了可注射水凝胶的溶胀性能、形态结构、力学强度、电化学性能、电导率、对宿主组织的粘附性和抗菌性能。水凝胶的电导率类似于10（-3）S.cm（-1），其对天然心脏组织相当大。C2 C12成肌细胞在水凝胶中的增殖显示了其良好的生物相容性。注射后，C2 C12细胞在水凝胶中的存活率与注射前无显著差异。两种不同类型的细胞被成功地封装在水凝胶的自愈效应。C2 C12成肌细胞和H9 c2心肌细胞的细胞递送曲线显示出可调的释放速率，并且还研究了导电水凝胶中的体内细胞保留。水凝胶的皮下注射和体内降解证明了其可注射性和生物降解性。总之，这些自愈导电可生物降解的可注射水凝胶是心脏修复的细胞递送载体的优秀候选者。
Cell therapy is a promising strategy to regenerate cardiac tissue for myocardial infarction. Injectable hydrogels with conductivity and self-healing ability are highly desirable as cell delivery vehicles for cardiac regeneration. Here, we developed self-healable conductive injectable hydro gels based on chitosan-graft-aniline tetramer (CS-AT) and dibenzaldehyde-terminated poly(ethylene glycol) (PEG-DA) as cell delivery vehicles for myocardial infarction. Self-healed electroactive hydrogels were obtained after mixing CS-AT and PEG-DA solutions at physiological conditions. Rapid self healing behavior was investigated by rheometer. Swelling behavior, morphology, mechanical strength, electrochemistry, conductivity, adhesiveness to host tissue and antibacterial property of the injectable hydrogels were fully studied. Conductivity of the hydrogels is similar to 10(-3) S.cm(-1), which is quite dose to native cardiac tissue. Proliferation of C2C12 myoblasts in the hydrogel showed its good biocompatibility. After injection, viability of C2C12 cells in the hydrogels showed no significant difference with that before injection. Two different cell types were successfully encapsulated in the hydrogels by self-healing effect. Cell delivery profile of C2C12 myoblasts and H9c2 cardiac cells showed a tunable release rate, and in vivo cell retention in the conductive hydrogels was also studied. Subcutaneous injection and in vivo degradation of the hydrogels demonstrated their injectability and biodegradability. Together, these self-healing conductive biodegradable injectable hydrogels are excellent candidates as cell delivery vehicle for cardiac repair.