Stability of Self-Assembled Polymeric Micelles in Serum.
Stability of Self-Assembled Polymeric Micelles in Serum.
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DOI：
10.1021/ma200675w
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发表时间：
2011-08-09
期刊：
影响因子：
5.5
通讯作者：
Shoichet MS
中科院分区：
文献类型：
--
作者：
Lu J;Owen SC;Shoichet MS
聚合物纳米颗粒在血清中的稳定性对于它们在药物递送中的使用是至关重要的，其中静脉注射后的稀释通常导致纳米颗粒分解和药物卸载;然而，很少有人在生物学相关介质中对此进行研究。为了更深入地了解血液中的纳米颗粒稳定性，在血清和单独的血清蛋白溶液中测试了聚（d，l-丙交酯-共-2-甲基-2-羧基三亚甲基碳酸酯）-g-聚（乙二醇），P（LA-共-TMCC）-g-PEG的自组装聚合物胶束的稳定性。通过封装Förster共振能量转移对并通过荧光释放它们，这些胶束在血清存在下表现出优异的热力学和动力学稳定性。通过快速蛋白质液相色谱和动态光散射的进一步分析证实了这些数据。此外，这些胶束与红细胞相容，如溶血测定所示。在血液中证明的稳定性和相容性表明这些胶束在体内可能是稳定的，这对于静脉内药物递送应用是至关重要的。这种理解胶束稳定性和相容性的综合方法具有广泛的适用性。
The stability of polymeric nanoparticles in serum is critical to their use in drug delivery where dilution after intravenous injection often results in nanoparticle disassembly and drug unloading; however, few investigate this in biologically relevant media. To gain greater insight into nanoparticle stability in blood, the stability of self-assembled polymeric micelles of poly(d,l-lactide-co-2-methyl-2-carboxytrimethylene carbonate)-g-poly(ethylene glycol), P(LA-co-TMCC)-g-PEG, were tested in both serum and individual serum protein solutions. By encapsulating Förster resonance energy transfer pairs and following their release by fluorescence, these micelles demonstrated excellent thermodynamic and kinetic stability in the presence of serum. Further analyses by fast protein liquid chromatography and dynamic light scattering confirmed these data. Moreover, these micelles are compatible with red blood cells, as shown by a hemolysis assay. The stability and compatibility demonstrated in blood suggest that these micelles may be stable in vivo, which is critical for intravenous drug delivery applications. This comprehensive approach to understanding micelle stability and compatibility is broadly applicable.
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影响因子：
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作者：
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通讯作者：
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作者：
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作者：
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DOI：
10.1073/pnas.0707046105
发表时间：
2008-05-06
影响因子：
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作者：
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