Serological AFP/Golgi protein 73 could be a new diagnostic parameter of hepatic diseases
Serological AFP/Golgi protein 73 could be a new diagnostic parameter of hepatic diseases
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DOI：
10.1002/ijc.25838
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发表时间：
2011-10-15
影响因子：
6.4
通讯作者：
Tian, Yaping
中科院分区：
文献类型：
--
作者：
Tian, Liyuan;Wang, Yu;Tian, Yaping
我们研究了GP73 （sGP73）的血清形态在不同肝脏病理过程中的变化规律，并确定了sGP73在炎症、纤维化和癌变中的作用，因为sGP73被认为是一种候选的肿瘤标志物。定量酶联免疫吸附法检测了535例肝细胞癌（HCC）、肝硬化（LC）、肝炎、局灶性结节增生（FNH）、血管瘤、肝内胆管癌（ICC）和腺癌转移癌（MC）患者的sGP73。LC患者的中位sGP73高于HCC和肝炎患者（p = 0.001），且三组患者的sGP73均高于健康人（p < 0.001）；Child-Pugh A级LC患者sGP73低于B、C级（p = 0.001），早期和晚期HCC组间无显著差异（110.4 μ g/L vs. 102.8 μ g/L）。AFP/GP73的敏感性为75.8%，特异性为79.7%，受者工作曲线下面积（AUROC）为0.844，而AFP的敏感性为95.2%，特异性为47.1% (p = 0.055)；检测早期HCC时，AFP/GP73 AUROC为0。AFP为804比0.766 （p = 0.086）。sGP73与LC中AST、AST/ALT、ALB、A/G和ALP相关。sGP73在血管瘤、FNH、ICC、MC中的阳性率分别为0、50、63.3、53.3%；AFP/GP73为0.796，区分MC与AFP阴性HCC的敏感性为81.4%，特异性为70.0%。sGP73升高与肝功能损害和慢性纤维化有关，与AFP联合可改善肝脏疾病的鉴别诊断。
We have investigated the changing rule of serum form of GP73 (sGP73) in different hepato-pathologic processes and identified the sGP73 role in inflammation, fibrosis and carcinogenesis since sGP73 has been regarded as a candidate tumor marker. Quantitative enzyme-linked immunosorbent assay detected sGP73 in 535 subjects with hepatocellular carcinoma (HCC), liver cirrhosis (LC), hepatitis, focal nodular hyperplasia (FNH), angioma, intra-hepatic cholangio-carcinoma (ICC) and metastatic cancer from adenocarcinomas (MC). Median sGP73 in LC was higher than in HCC and hepatitis (p = 0.001), and sGP73 in all three groups were higher than those in healthy individuals (p < 0.001); sGP73 in LC patients with Child-Pugh class A was lower than in class B and C (p = 0.001), no significant difference was found between early and advanced HCC groups (110.4 mu g/L vs. 102.8 mu g/L). AFP/GP73 had a sensitivity of 75.8% and specificity of 79.7% with an area under the receiver operating curve (AUROC) of 0.844 vs. 0.812 for AFP (p = 0.055) with a sensitivity of 95.2% and specificity of 47.1%; in detecting early HCC, AUROC of AFP/GP73 was 0. 804 vs. 0.766 for AFP (p = 0.086). sGP73 correlated with AST, AST/ALT, ALB, A/G and ALP in LC. The positive rate of sGP73 in angioma, FNH, ICC, and MC was 0, 50, 63.3, 53.3%, respectively; AFP/GP73 was 0.796 with the sensitivity of 81.4% and specificity of 70.0% when differentiating MC from AFP-negative HCC. Increased sGP73 is related to hepatic impairment and chronic fibrosis, and when combined with AFP could improve the differential diagnosis of hepatic diseases.