Characterization of resin in radiata pine woods, bisulfite pulps and mill pitch samples
Characterization of resin in radiata pine woods, bisulfite pulps and mill pitch samples
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发表时间：
1997
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通讯作者：
A. Wallis;R. H. Wearne
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作者：
A. Wallis;R. H. Wearne
介绍了辐射松（Pinus radiata）木材、亚硫酸盐纸浆和沥青样品中亲脂提取物（树脂）的色谱分析方法。从新鲜木材样品中提取的物质质量随着溶剂极性的减小而减小，其顺序为甲醇>丙酮>二氯甲烷>己烷。然而，分析的单个亲脂萃取物的数量并不依赖于萃取溶剂。高效排相色谱法（HPSEC）将提取物分为甘油三酯、甾醇酯、甾醇和脂肪酸、树脂酸四个部分。三甲基硅基（TMS）衍生物的气相色谱-质谱（GC-MS）对树脂酸和脂肪酸（TMS酯）和甾醇（TMS醚）进行了较好的分离。沥青样品以脂肪酸盐为主，树脂酸盐、甾醇和甾醇酯含量较少，甘油三酯含量较低。结果表明，气相色谱-质谱联用和高效液相色谱联用是分析辐射松提取物的有效方法，可以估计辐射松提取物中单个树脂、脂肪酸、甾体、甾醇酯和甘油三酯的总量。
Methods for the chromatographic analysis of lipophilic extractives (resin) in radiata pine (Pinus radiata) wood, bisulfite pulp and mill pitch samples are described. The mass of substances extracted from fresh wood samples decreased as the polarity of solvents decreased, in the order methanol > acetone > dichloromethane > hexane. However the amounts of individual lipophilic extractives analysed were not as dependent on extraction solvent. High performance size exclusion chromatography (HPSEC) separated the extracts into four fractions namely triglycerides, steryl esters, sterols and fatty acids, and resin acids. Gas chromatography-mass spectrometry (GC-MS) of trimethylsilyl (TMS) derivatives of the extracts gave good separation of resin acids and fatty acids as their TMS esters, and of sterols as their TMS ethers The pitch sample consisted mainly of salts of fatty acids, with smaller amounts of resin acid salts, sterols and steryl esters, and only trace amounts of triglycerides. It is concluded that the combination of GC-MS and HPSEC is useful for the analysis of radiata pine extractives, allowing the estimation of individual resin and fatty acids and steroids, and total amounts of steryl esters and triglycerides.