Plasma membrane inositol 1,4,5-trisphosphate receptor of lymphocytes: selective enrichment in sialic acid and unique binding specificity.
Plasma membrane inositol 1,4,5-trisphosphate receptor of lymphocytes: selective enrichment in sialic acid and unique binding specificity.
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淋巴细胞质膜肌醇1,4,5-三磷酸受体：唾液酸的选择性富集和独特的结合特异性。
DOI：
10.1073/pnas.89.7.2849
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发表时间：
1992
影响因子：
11.1
通讯作者：
Snyder,SH
中科院分区：
文献类型：
--
作者：
Khan,AA;Steiner,JP;Snyder,SH
与淋巴细胞质膜相关的肌醇1，4，5-三磷酸受体（IP 3R）在末端糖含量和结合特异性方面与位于内质网的小脑受体不同。凝集素柱层析显示，30%的IP 3R在胸腺中含有唾液酸，反映了质膜协会，相反，小脑IP 3R的5%。Jurkat淋巴细胞的胸腺和质膜组分中的IP 3R与Jurkat微粒体和小脑中的IP 3R在磷酸肌醇特异性上不同。质膜IP 3R对IP 3的亲和力较低，但对肌醇1，3，4，5-四磷酸的亲和力较高，这可能反映了肌醇磷酸对质膜钙的独特调节。
The inositol 1,4,5-trisphosphate receptor (IP3R) associated with plasma membranes of lymphocytes differs in terminal sugar content and binding specificity from the cerebellar receptor, which is localized to endoplasmic reticulum. Lectin column chromatography reveals that 30% of IP3R in the thymus contains sialic acid, reflecting a plasma membrane association, in contrast to 5% of cerebellar IP3R. IP3R in thymus and plasma membrane fractions of Jurkat lymphocytes differs from IP3R of Jurkat microsomes and cerebellum in inositol phosphate specificity. The plasma membrane IP3R has lower affinity for IP3 but higher affinity for inositol 1,3,4,5-tetrakisphosphate, which may reflect a unique regulation of calcium at the plasma membrane by inositol phosphates.