Bovine articular chondrocyte function in vitro depends upon oxygen tension
Bovine articular chondrocyte function in vitro depends upon oxygen tension
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DOI：
10.1053/joca.1999.0314
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发表时间：
2000-09-01
影响因子：
7
通讯作者：
Mason, RM
中科院分区：
文献类型：
--
作者：
Grimshaw, MJ;Mason, RM
关节软骨是一种生理性缺氧组织，其氧张力梯度从软骨表面约10%的氧到最深层的不到1%。软骨细胞在这个梯度内的位置可以调节细胞的行为和表型。此外，在关节疾病中，滑膜液的pO(2)下降，可能导致软骨细胞行为和基因表达的改变，从而可能干扰氧梯度。因此，有必要了解软骨细胞对不同氧张力的反应。我们比较了牛关节软骨细胞在海藻酸珠中培养7天的行为
Articular cartilage is a physiologically hypoxic tissue with a proposed gradient of oxygen tension ranging from about 10% oxygen at the cartilage surface to less than 1% in the deepest layers. The position of the chondrocyte within this gradient may modulate the cell's behavior and phenotype. Moreover, the oxygen gradient is likely to be disturbed during joint diseases in which the pO(2) of the synovial fluid declines which may cause changes in chondrocyte behavior and gene expression. Thus, there is a need to understand the chondrocyte's response to different oxygen tensions. We compared the behavior of bovine articular chondrocytes cultured in alginate beads for 7 days in medium maintained at