Reprogramming of adult human peripheral blood mononuclear cells into hiPSCs from two patients with facioscapulohumeral muscular dystrophy type 1.
Reprogramming of adult human peripheral blood mononuclear cells into hiPSCs from two patients with facioscapulohumeral muscular dystrophy type 1.
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DOI：
10.1016/j.scr.2022.102678
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发表时间：
2022-01
影响因子：
1.2
通讯作者：
Fu-ze Zheng;Long Chen;L. Qiu;Lin Lin-Lin;Xin Lin;Qifang He;Lili Wang;Zhi-xian Ye;M-T Lin;Zhiqiang Wang
中科院分区：
文献类型：
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作者：
Fu-ze Zheng;Long Chen;L. Qiu;Lin Lin-Lin;Xin Lin;Qifang He;Lili Wang;Zhi-xian Ye;M-T Lin;Zhiqiang Wang
Facioscapulohumeral muscular dystrophy (FSHD) is one of the most common muscular dystrophy. FSHD type 1 (FSHD1) is caused by multicopy contraction of D4Z4 repeats on chromosome 4q35. Human induced pluripotent stem cell (hiPSC) lines serve as important research models for various types of diseases in vitro. Here, we reprogrammed human peripheral blood mononuclear cells (PMBCs) into hiPSCs with episomal plasmid from two FSHD1 patients. These hiPSC lines maintained normal karyotype and exhibited typical morphology. Both of them could express pluripotency markers and differentiate into three layers. The hiPSC lines could be used for screening potential therapeutic targets and mechanism research.
