PROTEOMIC ANALYSIS OF NOVEL Cry1Ac BINDING PROTEINS IN Helicoverpa armigera (HUBNER)
PROTEOMIC ANALYSIS OF NOVEL Cry1Ac BINDING PROTEINS IN Helicoverpa armigera (HUBNER)
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DOI：
10.1002/arch.20340
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发表时间：
2010-02-01
影响因子：
2.2
通讯作者：
Rector，Brian G.
中科院分区：
文献类型：
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作者：
Chen，Li-Zhen;Liang，Ge-Mei;Rector，Brian G.
氨基肽酶N （APN）和钙粘蛋白样蛋白已被鉴定为棉铃虫cry1ac结合蛋白（Hubner）。本研究采用蛋白质组学方法鉴定了棉铃虫中新的cry1ac结合蛋白。采用双向凝胶电泳（2-DE）技术对棉铃虫刷状边缘膜泡进行了提取和分离。用抗Cry1Ac血清检测Cry1Ac结合蛋白。肽质量指纹图谱（PMF）用于鉴定cry1ac结合蛋白。总共鉴定出4种蛋白作为棉蚜cry1ac的候选结合蛋白：液泡ATP合成酶（V-ATPase）亚基B、肌动蛋白、热休克同源蛋白（HSCP）和一种新蛋白。C 2009 Wiley期刊公司
Aminopeptidase N (APN) and cadherin-like proteins have been previously identified as Cry1Ac-binding proteins in Helicoverpa armigera (Hubner). In this study, a proteomic approach was used to identify novel Cry1Ac-binding proteins in H. armigera. Brush border membrane vesicles (BBMV) of H. armigera were extracted and separated by two-dimensional gel electrophoresis (2-DE). Cry1Ac-binding proteins were detected using antisera against Cry1Ac. Peptide mass fingerprinting (PMF) was used to identify Cry1Ac-binding proteins. In total, four proteins were identified as candidate Cry1Ac-binding proteins in H. armigera: vacuolar ATP synthase (V-ATPase) subunit B, actin, heat shock cognate protein (HSCP), and a novel protein. C 2009 Wiley Periodicals, Inc.