Chronic treatment with nicotine or potassium attenuates depolarisation-evoked noradrenaline release from the human neuroblastoma SH-SY5Y
Chronic treatment with nicotine or potassium attenuates depolarisation-evoked noradrenaline release from the human neuroblastoma SH-SY5Y
复制标题
DOI：
10.1016/s0304-3940(02)00881-9
复制
发表时间：
2002-10-18
影响因子：
2.5
通讯作者：
Vaughan, PFT
中科院分区：
文献类型：
--
作者：
Agis-Torres, A;Ball, SG;Vaughan, PFT
用KCl（20 mM）慢性处理SH-SY 5 Y细胞4天，使100 mM KCl诱发的去甲肾上腺素（NA）释放减少50%，使尼古丁（100 μ M）诱发的NA释放减少55%。预处理与L-型钙通道拮抗剂，硝苯地平，防止这种抑制作用的慢性暴露于20 mM KCl对NA的释放。相反，用10 W尼古丁预处理4天对100 mM KCl诱发的分泌没有影响，并且仅使尼古丁诱发的NA释放减少25%。包括nifeclipine防止抑制NA释放的慢性尼古丁治疗。这些数据进行了讨论，慢性中度，去极化的K+或尼古丁对Ca 2+通过L-型电压敏感的钙通道流入的影响。(C)2002爱思唯尔科学爱尔兰有限公司保留所有权利。
Chronic treatment, of SH-SY5Y cells, with KCl (20 mM) for 4 days decreased 100 mM KCl-evoked noradrenaline (NA) release by 50% and nicotine (100 muM)-evoked NA release by 55%. Pretreatment with the L-type calcium channel antagonist, nifedipine, prevented this inhibitory effect of chronic exposure to 20 mM KCl on NA release. In contrast pretreatment with 10 W nicotine for 4 days had no effect on 100 mM KCl -evoked secretion and decreased nicotinic -evoked NA release by only 25%. Inclusion of nifeclipine prevented the inhibition of NA release by chronic nicotine treatment. These data are discussed in relation to effects of chronic moderate, depolarisation by either K+ or nicotine on influx of Ca2+ via L-type voltage sensitive calcium channels. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.