Genetic influence on carotid body structure in DBA/2J and A/J strains of mice.
Genetic influence on carotid body structure in DBA/2J and A/J strains of mice.
复制标题
DBA/2J 和 A/J 品系小鼠颈动脉体结构的遗传影响。
DOI：
10.1007/0-387-31311-7_16
复制
发表时间：
2006
影响因子：
--
通讯作者：
Shirahata,Machiko
中科院分区：
文献类型：
--
作者：
Yamaguchi,Shigeki;Balbir,Alexander;Okumura,Mariko;Schofield,Brian;Coram,Judith;Tankersley,ClarkeG;Fitzgerald,RobertS;O'Donnell,ChristopherP;Shirahata,Machiko
颈动脉体是动脉血中缺氧、高碳酸血症和酸中毒的主要化学感受器官（Fitzgerald和Shirahata 1997; Gonzalez等，1994年）。在缺氧期间，颈动脉体的神经输出增加，并反射性地改变呼吸系统中的几个变量。一个突出的反应是通气量的增加，但个体之间的低氧通气反应（HVR）变化很大（Eschele等人，1992; Vizek等人，1987年）。对人类的研究（柯林斯等人，1978; Kawakami等人，1982; Nishimura等人，1991;托马斯等人，1993）表明遗传因素对这些差异有很大的影响。类似地，在小鼠中的研究（坦克斯利等人，1994）和大鼠（Weil等人，1998）清楚地表明遗传决定因素强烈地影响HVR。在几种近交系小鼠中，DBA/2 J小鼠表现出最高的HVR，而A/J小鼠表现出最低的HVR（坦克斯利等人，1994年）。这两种品系小鼠之间的HVR差异可能与颈动脉体的结构差异密切相关（Yamaguchi et al.，2003年）。DBA/2 J小鼠颈动脉体的大小和血管球细胞的数量明显大于A/J小鼠。这些差异在品系之间明显分离，表明遗传因素强烈影响DBA/2 J和A/J小鼠之间观察到的表型差异。本研究的目的是证实DBA/2 J和A/J小鼠颈动脉体的形态特征差异是由遗传因素控制的。因此，我们通过将DBA/2 J（雌性）和A/J（雄性）小鼠品系杂交产生第一子代（F1），并检查DBA/2 J、A/J及其F1小鼠颈动脉体的形态学差异。
AbstractThe carotid body is a major chemosensory organ for hypoxia, hypercapnia and acidosis in the arterial blood (Fitzgerald and Shirahata 1997; Gonzalez et al., 1994). During hypoxia, the neural output from the carotid body increases and reflexly modifies several variables in the respiratory system. A prominent response is an increase in ventilation, but the hypoxic ventilatory response (HVR) among individuals varies widely (Eisele et al., 1992; Vizek et al., 1987). Studies in humans (Collins et al., 1978; Kawakami et al., 1982; Nishimura et al., 1991; Thomas et al., 1993) suggest that genetic factors significantly contribute to those differences. Similarly, studies in mice (Tankersley et al., 1994) and rats (Weil et al., 1998) clearly indicate that genetic determinants robustly influenced HVR. Among several inbred strains of mice the DBA/2J mice demonstrated the highest HVR and the A/J mice the lowest HVR (Tankersley et al., 1994). The differences in HVR between these two strains of mice may be closely related to the structural differences of the carotid body (Yamaguchi et al., 2003). The size of the carotid body and the quantity of glomus cells in the DBA/2J mice are significantly larger than those in the A/J mice. Those differences were clearly segregated between the strains, suggesting that genetic factors strongly influence the observed phenotypic differences between the DBA/2J and A/J mice. The purpose of the current study was to confirm that the morphological characteristic differences in the carotid body between the DBA/2J and A/J mice are controlled by genetic factors. Thus, we generated the first-filial progeny (F1) by a crossing the DBA/2J (female) and A/J (male) strains of mice, and examined the morphological differences of the carotid body in the DBA/2J, A/J and their F1 mice.