Establishment of human cytotoxic T cell lines specific for human adult T cell leukemia virus-bearing cells.
Establishment of human cytotoxic T cell lines specific for human adult T cell leukemia virus-bearing cells.
复制标题
建立对人类成人 T 细胞白血病病毒携带细胞具有特异性的人类细胞毒性 T 细胞系。
作者：
M. Kannagi;K. Sugamura;H. Sato;K. Okochi;H. Uchino;Y. Hinuma
体外培养了针对自体成人T细胞白血病病毒携带细胞特异性的人细胞毒性T细胞（Tc）。自体携带atlv的T细胞反复刺激健康供者外周血白细胞（PBL）。在四分之二的血清阳性供者和两分之一的血清阴性供者中，应答细胞对自身携带atlv的细胞表现出细胞毒性，但对自身PBL、自身克隆T细胞系、爱泼斯坦巴尔病毒转化的自体淋巴母细胞样B细胞系（LCL）细胞或各种atlv阴性细胞系细胞没有毒性。诱导的效应细胞是含有Leu-1和Leu-2a抗原的细胞毒性T细胞，通过适当细胞的周期性再刺激和补充部分纯化的T细胞生长因子（TCGF），这些细胞能够在体外连续增殖。在测试的三个Tc系中，一个对与效应细胞共享HLA-A2抗原的异体atlv承载细胞表现出明显的细胞毒性，可能表明hla限制atlv特异性Tc。冷靶抑制细胞毒性也提示hla限制。有趣的是，来自该个体的Tc也可以杀死不表达ATLV表面抗原的新鲜成人T细胞白血病细胞。这些结果，连同其他Tc系的靶特异性，表明atlv特异性Tc可能识别至少两种不同的靶抗原，其中一种抗原与血清学方法检测到的抗原不同。
Human cytotoxic T cells (Tc) specific for autologous adult T cell leukemia virus- (ATLV) bearing cells were induced in vitro. Peripheral blood leukocytes (PBL) from healthy donors were stimulated repeatedly with autologous ATLV-bearing T cells. In two out of four seropositive donors and one out of two seronegative donors, the responder cells showed cytotoxicity for autologous ATLV-bearing cells, but not for autologous PBL, autologous cloned T cell lines, Epstein Barr virus-transformed autologous lymphoblastoid B cell line (LCL) cells, or various ATLV-negative cell line cells. The effector cells induced were cytotoxic T cells possessing Leu-1 and Leu-2a antigens that were able to proliferate continuously in vitro with periodic restimulation by appropriate cells and supplementation with partially purified T cell growth factor (TCGF). Of three Tc lines tested, one exhibited significant cytotoxicity against allogeneic ATLV-bearing cells that shared HLA-A2 antigen with the effector cells, possibly indicating HLA-restriction of ATLV-specific Tc. HLA-restriction was also suggested by cold target inhibition of cytotoxicity. Interestingly, Tc from this individual could also kill fresh adult T cell leukemia cells that were not expressing the ATLV surface antigens. These results, together with the target specificities of other Tc lines, suggest the possibility of at least two distinct target antigens recognized by ATLV-specific Tc, with one of these antigens differing from the ones detected by serologic methods.