Size-dependent cytotoxicity of gold nanoparticles
Size-dependent cytotoxicity of gold nanoparticles
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DOI：
10.1002/smll.200700378
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发表时间：
2007-11-01
期刊：
影响因子：
13.3
通讯作者：
Jahnen-Dechent, Willi
中科院分区：
文献类型：
--
作者：
Pan, Yu;Neuss, Sabine;Jahnen-Dechent, Willi
金纳米颗粒广泛用于生物医学成像和诊断测试。基于它们在实验室中的既定用途和Au的化学稳定性，预计金纳米颗粒是安全的。然而，最近的文献中包含了关于金纳米颗粒细胞毒性的相互矛盾的数据。在此背景下，由三苯基膦衍生物稳定的水溶性金纳米粒子的尺寸范围从0.8到15 nm的系统研究。测试了这些颗粒在代表具有屏障和吞噬细胞功能的主要功能细胞类型的四种细胞系中的细胞毒性。结缔组织成纤维细胞、上皮细胞、巨噬细胞和黑色素瘤细胞证明对1.4 nm大小的金颗粒最敏感，这导致IC 50值范围为30至56 cm，取决于特定的1.4 nm Au化合物-细胞系组合。相比之下，15 nm大小的金颗粒和Tauredon（硫代苹果酸金）分别在高达60倍和100倍的浓度下是无毒的。细胞反应是大小依赖性的，在1.4 nm的颗粒主要导致快速细胞死亡的坏死在12小时内，而密切相关的颗粒1.2 nm的直径的效果主要程序性细胞死亡的细胞凋亡。
Gold nanoparticles are widely used in biomedical imaging and diagnostic tests. Based on their established use in the laboratory and the chemical stability of Au, gold nanoparticles were expected to be safe. The recent literature, however, contains conflicting data regarding the cytotoxicity of gold nanoparticles. Against this background a systematic study of watersoluble gold nanoparticles stabilized by triphenylphosphine derivatives ranging in size from 0.8 to 15 nm is made. The cytotoxicity of these particles in four cell lines representing major functional cell types with barrier and phagocyte function are tested. Connective tissue fibroblasts, epithelial cells, macrophages, and melanoma cells prove most sensitive to gold particles 1.4 nm in size, which results in IC50 values ranging from 30 to 56,cm depending on the particular 1.4-nm Au compound-cell line combination. In contrast, gold particles 15 nm in size and Tauredon (gold thiomalate) are nontoxic at up to 60-fold and 100-fold higher concentrations, respectively. The cellular response is size dependent, in that 1.4-nm particles cause predominantly rapid cell death by necrosis within 12 h while closely related particles 1.2 nm in diameter effect predominantly programmed cell death by apoptosis.