Liquid Extraction Surface Analysis Mass Spectrometry Coupled with Field Asymmetric Waveform Ion Mobility Spectrometry for Analysis of Intact Proteins from Biological Substrates
Liquid Extraction Surface Analysis Mass Spectrometry Coupled with Field Asymmetric Waveform Ion Mobility Spectrometry for Analysis of Intact Proteins from Biological Substrates
复制标题
DOI：
10.1021/acs.analchem.5b01151
复制
发表时间：
2015-07-07
影响因子：
7.4
通讯作者：
Cooper, Helen J.
中科院分区：
文献类型：
--
作者：
Sarsby, Joscelyn;Griffiths, Rian L.;Cooper, Helen J.
以前，我们已经表明，液体萃取表面分析（LESA）质谱法是适合于从一系列生物底物的完整蛋白质的分析。在这里，我们展示了LESA：质谱法可以与高场不对称波形离子迁移谱法（FAIMS）结合，用于直接从薄组织切片（小鼠肝脏、小鼠大脑）和从生长在琼脂上的细菌菌落（大肠杆菌）进行自上而下的蛋白质分析。PALMS的加入显著改善了信噪比并缩短了分析时间。在单扫描质谱中观察到丰富的蛋白质信号。此外，FAIMS能够实现分子类别（例如脂质和蛋白质）的气相分离，从而能够改进对来自单个LESA提取的两组物质的分析。
Previously we have shown that liquid extraction surface analysis (LESA) mass spectrometry is suitable for the analysis of intact proteins from a range of biological substrates. Here we show that LESA: mass spectrometry may be coupled with high field asymmetric waveform ion mobility spectrometry (FAIMS) for top-down protein analysis directly from thin tissue sections,(mouse liver, mouse brain) and from bacterial colonies (Escherichia coli) growing on agar. Incorporation of PALMS results in significant improvements in signal-to-noise and reduced analysis time. Abundant protein signals are observed in single scan mass spectra. In addition, FAIMS enables gas-phase separation of molecular classes, for example, lipids and proteins, enabling improved analysis of both sets of species from a single LESA extraction.