Situ Ultrahigh-resolution Optical Coherence Tomography Characterization of Eye Bank Corneal Tissue Processed for Lamellar Keratoplasty
Situ Ultrahigh-resolution Optical Coherence Tomography Characterization of Eye Bank Corneal Tissue Processed for Lamellar Keratoplasty
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DOI：
10.1097/ico.0b013e318169d6b7
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发表时间：
2008-08-01
期刊：
影响因子：
2.8
通讯作者：
Li, Xingde
中科院分区：
文献类型：
--
作者：
Brown, Jamin S.;Wang, Danling;Li, Xingde
目的：使用光学相干断层扫描 (OCT) 作为一种非侵入性方法，对用于板层角膜移植术的眼库角膜组织进行原位表征。方法：使用定制的超高分辨率 OCT (UHR-OCT) 对用于板层角膜移植术的供体角膜组织进行表征。对二十七个供体角膜进行了分析。四个供体角膜用作对照，而其余的则通过手解剖、微型角膜刀或飞秒激光加工成用于板层移植的供体角膜纽扣。 UHR-OCT 还用于无创地表征和监测浸入存储介质中超过 3 周的活角膜组织。结果：UHR-OCT 捕获了供体角膜组织的原位高分辨率图像。这种非侵入性技术显示了供体角膜组织形态在存储介质中随时间的变化。 UHR-OCT 清晰可见每种处理方式下的板层角膜组织的特征。飞秒激光切割皮质组织的原位表征注意到比常规组织学显示的界面碎片更多。在存储介质中，飞秒激光微空化气泡对角膜组织的影响在层状瓣的边缘处得到了很好的可视化。结论：我们的可行性研究的结果表明，UHR-OCT 可以无创地提供眼库角膜组织的极佳的病态微结构表征。 UHR-OCT 界面结果和角膜内皮盘厚度均匀性分析是有价值的信息，可用于优化层状组织处理的方式和参数。 UHR-OCT 是一种强大的方法，使 LIS 能够进一步评估组织对后板层角膜移植术不同处理技术的反应。它还可以提供可用于与术后临床结果相关的信息。 UHR-OCT 有潜力成为任何眼库或中心组织分析的常规部分，为移植定制板层角膜组织。
Purpose: To use optical coherence tomography (OCT) as a non-invasive too] to perform in situ characterization of eye bank corneal tissue processed for lamellar keratoplasty.Methods: A custom-built ultrahigh-resolution OCT (UHR-OCT) was used to characterize donor corneal tissue that had been processed for lamellar keratoplasty. Twenty-seven donor corneas were analyzed. Four donor corneas were used as controls, whereas the rest were processed into donor corneal buttons for lamellar transplantation by using hand dissection, a microkeratome, or a femtosecond laser. UHR-OCT was also used to noninvasively characterize and monitor the viable corneal tissue immersed in storage medium over 3 weeks.Results: The UHR-OCT captured high-resolution images of the donor corneal tissue in situ. This noninvasive technique showed the changes in donor corneal tissue morphology with time while in storage medium. The characteristics of the lamellar corneal tissue with each processing modality were clearly visible by UHR-OCT. The in Situ characterization of the femtosecond laser-cut cortical tissue was noted to have more interface debris than shown by routine histology. The effects of the femtosecond laser microcavitation bubbles oil the corneal tissue were well Visualized at the edges of the lamellar flap while in storage medium.Conclusions: The results of our feasibility stud), show that UHR-OCT can provide Superb, ill Situ microstructural characterization of eye bank corneal tissue noninvasively. The UHR-OCT interface findings and corneal endothelial disc thickness uniformity analysis are valuable information that may be used to optimize the modalities and parameters for lamellar tissue processing. The UHR-OCT is a powerful approach that will allow LIS to further evaluate the tissue response to different processing techniques for posterior lamellar keratoplasty. It may also provide information that can be used to correlate with postoperative clinical outcomes. UHR-OCT has the potential to become a routine part of tissue analysis for any eye bank or centers creating customized lamellar corneal tissue for transplantation.