Characterization of transposon Tn1549, conferring VanB-type resistance in Enterococcus spp.
Characterization of transposon Tn1549, conferring VanB-type resistance in Enterococcus spp.
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DOI：
10.1099/00221287-146-6-1481
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发表时间：
2000-06-01
期刊：
影响因子：
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通讯作者：
Galimand, M
中科院分区：
文献类型：
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作者：
Garnier, F;Taourit, S;Galimand, M
据报道，肠球菌中VanB型糖肽耐药性的转移与大染色体遗传元件或质粒的移动有关。作者报道了肠球菌属临床分离株中pAD 1相关质粒携带的34 kb转座子Tn 1549的特征，该转座子赋予万古霉素耐药性。Tn 1549包含30个ORF，并且看起来像Tn 916接合转座子家族一样被组织成三个功能区域：（i）右端，涉及切除-整合过程;（ii）中心部分，其中vanB 2操纵子取代泰特（M）基因;和（iii）左端，其中18个ORF中的8个可能涉及接合转移。
Transfer of VanB-type resistance to glycopeptides among enterococci has been reported to be associated with the movement of large chromosomal genetic elements or of plasmids. The authors report the characterization of the 34 kb transposon Tn1549 borne by a plasmid related to pAD1 and conferring vancomycin resistance in clinical isolates of Enterococcus spp. Tn1549 contained 30 ORFs and appeared to be organized like the Tn916 family of conjugative transposons into three functional regions: (i) the right end, implicated in the excision-integration process; (ii) the central part, in which the vanB2 operon replaces the tet(M) gene; and (iii) the left extremity, in which eight of the 18 ORFs could be implicated in the conjugative transfer.