Brain stem mechanisms underlying acupuncture modality-related modulation of cardiovascular responses in rats.
Brain stem mechanisms underlying acupuncture modality-related modulation of cardiovascular responses in rats.
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DOI：
10.1152/japplphysiol.01365.2004
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发表时间：
2005-09
影响因子：
3.3
通讯作者：
Wei Zhou;S. Tjen-A-Looi;J. Longhurst
中科院分区：
文献类型：
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作者：
Wei Zhou;S. Tjen-A-Looi;J. Longhurst
本研究旨在观察正中神经上方心包P(5-6)穴不同频率的手针(MA)和电针(EA)对脑干的反应。本文记录了机械麻醉大鼠在刺激内脏和躯体传入时，延髓头端腹外侧区(RVLM)运动前交感心血管神经元的活动。我们用电针(0.3~0.5 mA，0.5ms)刺激内脏神经(0.1~0.4 mA，0.5ms)或以大约2 Hz(MA)刺激S正中神经30例。在对内脏神经和正中神经刺激有反应的18个细胞中，有12个细胞可从胸髓中外侧柱(T2-T4)逆行驱动，表明它们是运动前交感神经元。所有18个神经元都接受了压力感受器的输入，这为它们的心血管交感兴奋功能提供了证据。刺激内脏神经的诱发反应，电针抑制49+/-6%(n=7)，MA抑制46+/-4%(n=6)，表明两种方式的抑制程度相似。电针或电针终止后，抑制作用持续20min。心血管运动前RVLM神经元对P5~6的2 Hz电刺激有反应，对10、20、40和100 Hz刺激的反应程度较小(53+/-10、16+/-2、8+/-2、2+/-1和0+/-0脉冲/30刺激，n=7)。这些结果表明，在低频电针和电针时，接受内脏神经和正中神经汇聚输入的RVLM运动前交感神经元同样被低频电针和电针抑制较长时间。
The present study was designed to investigate brain stem responses to manual acupuncture (MA) and electroacupuncture (EA) at different frequencies at pericardial P (5-6) acupoints located over the median nerve. Activity of premotor sympathetic cardiovascular neurons in the rostral ventral lateral medulla (rVLM) was recorded during stimulation of visceral and somatic afferents in ventilated anesthetized rats. We stimulated either the splanchnic nerve at 2 Hz (0.1-0.4 mA, 0.5 ms) or the median nerve for 30 s at 2, 10, 20, 40, or 100 Hz using EA (0.3-0.5 mA, 0.5 ms) or at approximately 2 Hz with MA. Twelve of 18 cells responsive to splanchnic and median nerve stimulation could be antidromically driven from the intermediolateral columns of the thoracic spinal cord, T2-T4, indicating that they were premotor sympathetic neurons. All 18 neurons received baroreceptor input, providing evidence of their cardiovascular sympathoexcitatory function. Evoked responses during stimulation of the splanchnic nerve were inhibited by 49 +/- 6% (n = 7) with EA and by 46 +/- 4% (n = 6) with MA, indicating that the extent of inhibitory effects of the two modalities were similar. Inhibition lasted for 20 min after termination of EA or MA. Cardiovascular premotor rVLM neurons responded to 2-Hz electrical stimulation at P 5-6 and to a lesser extent to 10-, 20-, 40-, and 100-Hz stimulation (53 +/- 10, 16 +/- 2, 8 +/- 2, 2 +/- 1, and 0 +/- 0 impulses/30 stimulations, n = 7). These results indicate that rVLM premotor sympathetic cardiovascular neurons that receive convergent input from the splanchnic and median nerves during low-frequency EA and MA are inhibited similarly for prolonged periods by low-frequency MA and EA.