Seroprevalence and risk factors of glanders in working equines - Findings of a cross-sectional study in Punjab province of Pakistan
Seroprevalence and risk factors of glanders in working equines - Findings of a cross-sectional study in Punjab province of Pakistan
复制标题
DOI：
10.1016/j.actatropica.2017.07.031
复制
发表时间：
2017-12-01
期刊：
影响因子：
2.7
通讯作者：
Jayarao, Bhushan M.
中科院分区：
文献类型：
--
作者：
Ghori, Muhammad Taslim;Khan, Muhammad Sarwar;Jayarao, Bhushan M.
腺病是马的一种传染性细菌性疾病。在该病流行的国家，对其在工作马中的血清流行率和危险因素知之甚少。此外，在先前有证据表明土壤中检测到伯克霍尔德菌（B. mallei）的地区，没有关于该病流行的报告。在2014-2015年期间，在巴基斯坦旁遮普省选定的地区（n = 09）进行了横断面研究。共筛选1008份血清样本，采用补体固定试验和western blot检测马氏双歧杆菌抗体。总血清阳性率为3.17% （95% CI: 2.25-4.44）。土壤中有马利弧菌的采样点血清阳性率显著高于其他采样点[OR: 10.66 (95% CI: 4.42 ~ 31.66), p = 0.00]。其他与动物血清阳性显著相关的危险因素有：年龄组[OR: 1.78 (95% CI: 4.58 ~ 15.56), p = 0.00]、城市地区[OR: 2.99 (95% CI: 1.46 ~ 6.51), p = 0.00]、身体状况[OR: 3.47 (95% CI: 1.64 ~ 7.99), p = 0.00]、是否存在严重病变[OR: 7.71 (95% CI: 3.47 ~ 19.50), p = 0.00]、靠近水体[OR: 7.71 (95% CI: 3.47 ~ 19.50), p = 0.00]；家畜种群[OR: 3.20 (95%CI: 1.24-10.87), p = 0.03]和抽样地区家庭数[OR: 4.18 (95%CI: 1.82-11.30), p = 0.00]。该研究提供了腺状杆菌流行率的估计，以及动物血清阳性与土壤中马氏杆菌存在之间的潜在联系。本研究确定的危险因素可用于监测和疾病认识。在发展中国家，拉车马匹中疾病的高流行率以及受感染的动物与其照料者的接触表明需要开始采用一种卫生方法逐步控制该疾病。
Glanders is an infectious and contagious bacterial disease of equines. A little is known about its seroprevalence and risk factors in working equines in countries where the disease is endemic. Also, there are no reports on prevalence of the disease in areas where there is a prior evidence of Burkholderia (B.) mallei detection in soil. A cross-sectional study was conducted in selected districts (n = 09) of Punjab province of Pakistan during 2014-2015. A total of 1008 serum samples were screened for detection of antibodies to B. mallei with complement fixation test followed by western blot. The overall seroprevalence was found to be 3.17% (95% CI: 2.25-4.44). The seropositivity was significantly higher from the sampling sites where B. mallei was detected in soil [OR: 10.66 (95% CI: 4.42-31.66), p = 0.00]. Other risk factors significantly associated with animal seropositivity were: age group [OR: 1.78 (95% CI: 4.58-15.56), p = 0.00], location in urban area [OR: 2.99 (95% CI: 1.46-6.51), p = 0.00],body condition [OR: 3.47 (95% CI: 1.64-7.99), p = 0.00], presence of farcy lesion [OR: 7.71 (95% CI: 3.47-19.50), p = 0.00], proximity to water bodies [OR: 7.71 (95% CI: 3.47-19.50), p = 0.00]; domestic animal population [OR: 3.20 (95% CI: 1.24-10.87), p = 0.03] and number of households in sampling area [OR: 4.18 (95%CI: 1.82-11.30), p = 0.00]. The study provides an estimate of prevalence of glanders and a potential link between animal seropositivity and presence of B. mallei in soil. The risk factors identified in this study can be used in surveillance and disease awareness. The high prevalence of disease in draught horses and contact of infected animals with their care-takers in developing countries signify need to initiate progressive control of the disease using one health approach.