Corynebacterium marinum sp nov isolated from coastal sediment
Corynebacterium marinum sp nov isolated from coastal sediment
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从沿海沉积物中分离出的海棒状杆菌新属
DOI：
10.1099/ijs.0.018523-0
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发表时间：
2010-08-01
影响因子：
2.8
通讯作者：
Austin, Brian
中科院分区：
文献类型：
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作者：
Du, Zong-Jun;Jordan, Elizabeth M.;Austin, Brian
对从青岛某燃煤电厂附近海域沉积物中分离到的一株细菌D 7015（T）进行了分类学研究。菌株D 7015（T）的细胞是革兰氏阳性的、非运动的、类白喉杆菌，其在0-8%（w/v）NaCl存在下和4-37 ℃下生长，在1%（w/v）NaCl和30-32 ℃下生长最佳。DNA的G+C含量为65.0mol%。主要脂肪酸为C-18：1 omega 9 c（56.18%）、C-16：0（38.02%）、C-16：1 omega 7 c（4.45%）、C-18：0（1.0%）和C-14：0（0.35%）。根据形态特征、生理生化特征和系统发育特征，将菌株D 7015（T）归属于棒状杆菌属（Corynebacterium）。与耐盐棒杆菌DSM 44683（T）的16 S rRNA基因序列相似性为95.9%，DNA相关性为20.4%。菌株D 7015（T）与棒状杆菌属的公认种有足够的不同，被认为是代表新种。拟命名为海洋棒杆菌属（Corynebacterium marinum sp. nov.），模式菌株为菌株D 7015（T）（=CGMCC 1.6998（T）=NRRL B-24779（T））。
A taxonomic study was performed on strain D7015(T), which was isolated from coastal sediment close to a coal-fired power station in Qingdao, China. Cells of strain D7015(T) were Gram-positive, non-motile, diphtheroid rods that grew in the presence of 0-8% (w/v) NaCl and at 4-37 degrees C, with optimum growth at 1 % (w/v) NaCl and 30-32 degrees C. The DNA G+C content was 65.0 mol%. The major fatty acids were C-18:1 omega 9c (56.18%), C-16:0 (38.02%), C-16:1 omega 7c (4.45%), C-18:0 (1.0%) and C-14:0 (0.35%). On the basis of morphological, physiological and phylogenetic characteristics, strain D7015(T) was classified in the genus Corynebacterium. It exhibited a 16S rRNA gene sequence similarity of 95.9% and a DNA DNA relatedness value of 20.4% with Corynebacterium halotolerans DSM 44683(T). Strain D7015(T) was sufficiently different from recognized species of the genus Corynebacterium to be considered to represent a novel species. The name Corynebacterium marinum sp. nov. is proposed, with strain D7015(T) (=CGMCC 1.6998(T)=NRRL B-24779(T)) as the type strain.