Abscisic acid induces heat tolerance in chickpea (Cicer arietinum L.) seedlings by facilitated accumulation of osmoprotectants
Abscisic acid induces heat tolerance in chickpea (Cicer arietinum L.) seedlings by facilitated accumulation of osmoprotectants
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DOI：
10.1007/s11738-012-0959-1
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发表时间：
2012-09-01
影响因子：
2.6
通讯作者：
Gaur, P.
中科院分区：
文献类型：
--
作者：
Kumar, Sanjeev;Kaushal, Neeru;Gaur, P.
全球气温的逐渐上升是农作物生长发育的主要问题。鹰嘴豆（Cicer arietinum L.）是一种对热敏感的作物，因此在其营养和生殖阶段都会受到损害。脱落酸（阿坝）是一种与胁迫相关的激素，具有耐热性，但其作用机制尚不完全清楚，尤其是是否与渗透调节物质（如脯氨酸、甜菜碱和海藻糖）有关。渗透调节物质也通过多种机制在保护植物免受热胁迫的伤害方面发挥重要作用。在本研究中，我们研究了阿坝和渗透调节剂在不同温度（30/25 A ℃（对照），35/30，40/35和45/40 A ℃）下水培生长的鹰嘴豆植物中的相互作用（白天/夜晚（12小时/12小时））：（a）不存在阿坝;（B）存在阿坝;和（c）存在其生物合成抑制剂氟啶酮（FLU）。研究结果表明，在45/40摄氏度的严重生长抑制，这是与内源性阿坝和渗透调节物质的急剧减少相比，未受胁迫的植物，这表明它们之间可能的关系。在40/35和45/40 ℃下，外源阿坝（2.5 μ M）显著抑制了幼苗的生长，而FLU的应用则加剧了抑制作用。阿坝在胁迫温度下的增长与阿坝和渗透调节物质的内源性水平的提高有关，而FLU则抑制了这一点。ABA处理的植物经历了少得多的氧化损伤测量丙二醛和过氧化氢含量。脯氨酸，甘氨酸甜菜碱和海藻糖（10 μ M）的外源应用也促进了热胁迫植物的生长，它们的作用与FLU应用没有显着影响，这表明这些渗透调节剂的功能下游的阿坝，部分介导这种激素的保护作用。
The gradual rise of global temperature is of major concern for growth and development of crops. Chickpea (Cicer arietinum L.) is a heat-sensitive crop and hence experiences damage at its vegetative and reproductive stages. Abscisic acid (ABA), a stress-related hormone, is reported to confer heat tolerance, but its mechanism is not fully known, especially whether it involves osmolytes (such as proline, glycine betaine and trehalose) in its action or not. Osmolytes too have a vital role in saving the plants from injurious effects of heat stress by multiple mechanisms. In the present study, we examined the interactive effects of ABA and osmolytes in chickpea plants grown hydroponically at varying temperatures of 30/25A degrees C (control), 35/30, 40/35 and 45/40A degrees C (as day/night (12 h/12 h)): (a) in the absence of ABA; (b) with ABA; and (c) in the presence of its biosynthetic inhibitor fluridone (FLU). The findings indicated severe growth inhibition at 45/40A degrees C that was associated with drastic reduction in endogenous ABA and osmolytes compared to the unstressed plants suggesting a possible relationship between them. Exogenous application of ABA (2.5 mu M) significantly mitigated the seedling growth at 40/35 and 45/40A degrees C, while FLU application intensified the inhibition. The increase in growth by ABA at stressful temperature was associated with enhancement of endogenous levels of ABA and osmolytes, while this was suppressed by FLU. ABA-treated plants experienced much less oxidative damage measured as malondialdehyde and hydrogen peroxide contents. Exogenous application of proline, glycine betaine and trehalose (10 mu M) also promoted the growth in heat-stressed plants and their action was not significantly affected with FLU application, suggesting that these osmolytes function downstream of ABA, mediating partially the protective effect of this hormone.